Abstract
Submerged culturing of mycelium is an efficient technique used to increase biomass yields, more so when employed with naturally slow-growing species of mushrooms. This paper is concerned with optimizing nutrient broth components used in Inonotus obliquus cultures for achieving high biomass yields. We modeled the effect of seven biotechnological parameters (six broth ingredients and the initial pH of nutritive broth) on mycelial biomass and predicted an optimum broth formula using response surface methodology. An analysis of variance showed that the elaborated model is significant (F-value of 2.76 and p-value of 0.0316). We used bioreactor cultures to confirm the model’s optimum prediction and to compare these results with a general-purpose mycology medium, namely potato dextrose broth (PDB). The optimized bioreactor culture yielded 4.37 g/L (93.36% of the dry weight prediction), while the PDB bioreactor culture yielded 2.084 g/L, after 15 days of cultivation. The optimized formula was: 2.15299 g malt extract, 3.99296 g yeast extract, 11.0041 g fructose, 17.4 g soluble starch, 0.1 g MgSO4, and 0.05 g CaCl2 per liter of broth.
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
Reference18 articles.
1. The use of mushroom glucans and proteoglycans in cancer treatment;Kidd;Altern. Med. Rev.,2000
2. Antitumor and Antiviral Substances from Fungi
3. Optimization of extraction process of crude polysaccharides from wild edible BaChu mushroom by response surface methodology
4. Medium Optimization for Polysaccharide Production of Cordyceps sinensis
5. Optimization of sub-merged culture conditions for biomass production in Pleurotus florida (Mont.) Singer, a Nigerian edible fungus;Jonathan;Afr. J. Biotechnol.,2006
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献