Metabolomic Diversity of Human Milk Cells over the Course of Lactation—A Preliminary Study-Reference-Cited by-同舟云学术

Metabolomic Diversity of Human Milk Cells over the Course of Lactation—A Preliminary Study

Published:2023-02-22 Issue:5 Volume:15 Page:1100
ISSN:2072-6643
Container-title:Nutrients
language:en
Short-container-title:Nutrients

Author:

Ten-Doménech Isabel¹^ORCID,Cascant-Vilaplana Mari Merce¹^ORCID,Navarro-Esteve Víctor¹²^ORCID,Felderer Birgit¹³,Moreno-Giménez Alba¹,Rienda Iván⁴^ORCID,Gormaz María¹⁵,Moreno-Torres Marta⁶⁷⁸^ORCID,Pérez-Guaita David²,Quintás Guillermo⁹,Kuligowski Julia¹^ORCID

Affiliation:

1. Neonatal Research Group, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain

2. Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Spain

3. Master Program Biotechnical Processes, Austrian Biotech University of Applied Sciences, Konrad Lorenz-Strasse 10, 3430 Tulln, Austria

4. Servicio de Anatomía Patológica, University & Polytechnic Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain

5. Division of Neonatology, University & Polytechnic Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain

6. Unidad de Hepatología Experimental y Trasplante Hepático, Health Research Institute Hospital La Fe, Avda Fernando Abril Martorell 106, 46026 Valencia, Spain

7. Department of Biochemistry and Molecular Biology, University of Valencia, C/Blasco Ibáñez 15, 46010 Valencia, Spain

8. Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Instituto de Salud Carlos III, 28220 Madrid, Spain

9. Health and Biomedicine, Leitat Technological Center, Carrer de la Innovació, 2, 08225 Terrassa, Spain

Abstract

Human milk (HM) is a complex biofluid containing a wide cell variety including epithelial cells and leukocytes. However, the cellular compositions and their phenotypic properties over the course of lactation are poorly understood. The aim of this preliminary study was to characterize the cellular metabolome of HM over the course of lactation. Cells were isolated via centrifugation and the cellular fraction was characterized via cytomorphology and immunocytochemical staining. Cell metabolites were extracted and analyzed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC–QqTOF-MS) in the positive and negative electrospray ionization modes. Immunocytochemical analysis revealed a high variability of the number of detected cells with relative median abundances of 98% of glandular epithelial cells, 1% of leukocytes, and 1% of keratinocytes. Significant correlations between the milk postnatal age with percentage of epithelial cells and leukocytes, and with total cell count were observed. Results from the Hierarchical Cluster Analysis of immunocytochemical profiles were very similar to those observed in the analysis of the metabolomic profiles. In addition, metabolic pathway analysis showed alterations in seven metabolic pathways correlating with postnatal age. This work paves the way for future investigations on changes in the metabolomic fraction of the cellular compartment of HM.

Funder

Generalitat Valenciana

Instituto de Salud Carlos III

Ministerio de Ciencia e Innovación

Publisher

MDPI AG

Subject

Food Science,Nutrition and Dietetics

Link

https://www.mdpi.com/2072-6643/15/5/1100/pdf

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