Abstract
Previous studies have shown that spermidine (Spd) can improve tolerance to high temperature stress in tomato seedlings. To further understand how Spd regulates the molecular components of high temperature stress signaling pathways, we performed a genome-wide transcriptome analysis in tomato seedlings treated with high temperature and/or exogenous Spd. The results demonstrate that, under high temperature conditions, Spd significantly alleviated the inhibition of plant growth, as well as improving the net photosynthetic rate and pigment contents. The transcriptome analysis revealed thousands of differentially expressed genes (DEGs) in response to high temperature with or without Spd treatment. Half of the genes were induced by high temperature, part of the genes were induced by high temperature with exogenous Spd, and some were induced by the coordinated effect of high temperature and Spd. A GO analysis indicated that genes involved in cellular processes, metabolic processes, and nucleotide binding in the sample were subjected to high temperature. Some DEGs were also involved in plant physiological processes. These results suggest potential genes and molecular pathways were involved in the exogenous Spd-mediated tolerance to high temperature stress in tomato plants. A JA signaling test was designed, which indicated that MYC2 and JAS1 in heat-resistant materials were both increased, through quantitative RT-PCR.
Funder
National Natural Science Foundation of China
China Agriculture Research System
Subject
Agronomy and Crop Science
Reference60 articles.
1. Plant tolerance to high temperature in a changing environment: Scientific fundamentals and production of heat stress-tolerant crops;Bita;Front. Plant Sci.,2013
2. Molecular responses of plants to cold shock and cold acclimation;Guy;J. Mol. Microbiol. Biotechnol.,1999
3. Plants and high temperature stress;Weis;J. Symp. Soc. Exp. Biol.,1988
4. Gruissem, W., Buchannan, B., and Jones, R. (2000). Biochemistry and Molecular Biology of Plants, Responses to Abiotic Stresses, American Society of Plant Physiologists.
5. Molecular genetics of heat tolerance and heat shock proteins in cereals;Maestri;Plant Mol. Biol.,2002
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献