Affiliation:
1. College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
2. Chengdu Juannong Intelligent Agriculture Technology Development Co., Ltd., Chengdu 611730, China
3. Department of Biochemistry, Baoding University, Baoding 071000, China
Abstract
Seed vigor is a crucial indicator comprehensively assessing the quality of seeds, reflecting the growth advantage and production potential of seeds, and has a significant effect on seeds’ stress resistance. Identifying and controlling loci related to wheat seed vigor is essential for accelerating genetic trait gains. Here, we performed a large genome-wide association study (GWAS) to identify several significant quantitative trait loci (QTLs) associated with seed vigor-related traits. A total of 404 wheat samples with diverse genetic backgrounds were used as experimental materials. Twenty-eight loci significantly associated with seed vigor-related traits in wheat were identified, distributed on chromosomes 3A, 4A, 5B, 7A, and 7B. Two potential novel loci controlling wheat seed vigor were discovered, with a total of 80 candidate genes associated with seed vigor located on these loci. Among them, TraesCS4A01G020000.1 encodes a late embryogenesis abundant (LEA) protein gene, and TraesCS5B01G298500.1 encodes a helicase gene, both showing specific expression in seeds and highly correlated with seed vigor. Overall, these findings provide valuable insights for the future application of these genes in wheat breeding.
Funder
Chengdu Key R&D Support Plan
School Enterprise Cooperation Plan
Reference27 articles.
1. Yang, M., Yang, Z., Yang, W., and Yang, E. (2022). Genetic Diversity Assessment of the International Maize and Wheat Improvement Center and Chinese Wheat Core Germplasms by Non-Denaturing Fluorescence In Situ Hybridization. Plants, 11.
2. Association of Official Seed Analysts (1983). Seed Vigor Testing Handbook, Association of Official Seed Analysts.
3. Seed germination and vigor;Rajjou;Annu. Rev. Plant Biol.,2012
4. A simple method to determine amino acid leakage and germination capabilities from single radish (Raphanus sativus L.) and Chinese cabbage (Brassica rapa ssp. pekinensis) seeds;Tai;Hortic. Environ. Biotechnol.,2013
5. Seed vigour and crop establishment: Extending performance beyond adaptation;Bassel;J. Exp. Bot.,2016