The 13C Discrimination of Crops Identifies Soil Spatial Variability Related to Water Shortage Vulnerability

Abstract

Spatial variability of crop growth and yields is the result of many interacting factors. The contribution of the factors to variable yields is often difficult to separate. This work studied the relationships between the 13C discrimination (Δ13C) of plants and the spatial variability of field soil conditions related to impacts of water shortage on crop yield. The 13C discrimination, the indicator of water shortage in plants, 15N (δ15N) discrimination, and nitrogen (N) content were determined in grains of winter wheat, spring barley, and pea. The traits were observed at several dozens of grid spots in seven fields situated in two regions with different soil and climate conditions between the years 2017 and 2019. The principles of precision agriculture were implemented in some of the studied fields and years by variable rate nitrogen fertilization. The Δ13C significantly correlated with grain yields (correlation coefficient from 0.66 to 0.94), with the exception of data from the wetter year 2019 at the site with higher soil water capacity. The effect of drought was demonstrated by statistically significant relationships between Δ13C in dry years and soil water capacity (r from 0.46 to 0.97). The significant correlations between Δ13C and N content of seeds and soil water capacity agreed with the expected impact of water shortage on plants. The 13C discrimination of crop seeds was confirmed as a reliable indicator of soil spatial variability related to water shortage. Stronger relationships were found in variably fertilized areas.