Genome-Wide Identification and Characterization of Glutaredoxin Family Genes in Common Wheat

Abstract

Glutaredoxins (GRXs) are small-molecular-weight proteins present in a wide range of organisms, and they play a key role in maintaining the redox homeostasis of cells. Most studies on GRXs are conducted in animals and humans, and those conducted on plants are scarce. The number and types of GRX genes vary in different plants. According to the active sites, the GRX family can be further divided into the CPYC, CGFS, and CC subfamilies. The CPYC and CGFS subfamilies are present in eukaryotes. The CC subfamily is exclusively present in higher plants and has the highest number of genes. In this study, 85 GRX genes were identified in common wheat (Triticum aestivum L.) using a bioinformatic method, wherein 12, 9, and 64 belonged to the CPYC, CGFS, and CC subfamilies, respectively. All TaGRX genes were homogeneously distributed in the three subgenomes of wheat. The gene structure analysis revealed that TaGRX members had 1–7 introns. The conserved motif analysis revealed that members of the same TaGRX subfamily had similar motifs. An analysis of cis-regulatory elements of promoters demonstrated that most TaGRX members had auxin-responsive elements; cis-regulatory elements, such as methyl jasmonate (MeJA), MYB, and abscisic acid (ABA), were distributed in all subfamilies, and the cell-cycle regulation element was only observed in the CC and CPYC subfamily members. Additionally, the synteny of the GRX genes in wheat, in wheat and Arabidopsis, and in wheat and barley was analyzed to clarify the evolutionary correlation of TaGRXs. The expression characteristics of TaGRXs were investigated, and TaGRX expression in various tissues and its responses to different abiotic stresses were preliminarily determined. This study provides a reference for the functional analysis of TaGRXs and understanding their role in molecular breeding of wheat.