An Integration of Transcriptomic Data and Modular Gene Co-Expression Network Analysis Uncovers Drought Stress-Related Hub Genes in Transgenic Rice Overexpressing OsAbp57

Abstract

Auxin receptor plays a significant role in the plant auxin signalling pathway in response to abiotic stress. Recently, we found that transgenic rice overexpressing ABP57 had higher drought tolerance than the wild-type cultivar, MR219, due to the fact of its enhanced leaf photosynthetic rate and yields under drought stress. We performed a microarray study on this line to investigate the underlying mechanisms contributing to the observed phenotype. After microarray data filtering, 3596 genes were subjected to modular gene co-expression network (mGCN) development using CEMiTool, an R package. We identified highly related genes in 12 modules that could act to specific responses towards drought or any of the abiotic stress types. Gene set enrichment and overrepresentation analyses for modules extracted two highly upregulated modules that are involved in drought-related biological processes such as transmembrane transport of metal ions and response to oxidative stress. Finally, 123 hub genes were identified in all modules after integrating co-expression information with physical interaction data. In addition, the interplay of significant pathways between the metabolism of chlorophyll and flavonoid and the signalling pathways of MAPK, IAA, and SA inferred the concurrent involvement of stress tolerance response. Collectively, our findings seek new future directions for breeding strategies in rice tolerant improvements.