Creation of Bacterial Blight Resistant Rice by Targeting Homologous Sequences of Xa13 and Xa25 Genes-Reference-Cited by-同舟云学术

Creation of Bacterial Blight Resistant Rice by Targeting Homologous Sequences of Xa13 and Xa25 Genes

Published:2024-04-12 Issue:4 Volume:14 Page:800
ISSN:2073-4395
Container-title:Agronomy
language:en
Short-container-title:Agronomy

Author:

Zhu Yiwang¹²,Yang Xiaohuai³,Luo Peirun³,Yan Jingwan¹,Cao Xinglan²⁴,Qian Hongge²^ORCID,Zhu Xiying²⁴,Fan Yujin²⁴,Mei Fating¹,Fan Meiying¹,Shang Lianguang²,Wang Feng¹,Zhang Yu⁵^ORCID

Affiliation:

1. Institute of Biotechnology, Fujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory of Genetic Engineering for Agriculture, Fuzhou 350003, China

2. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China

3. Shenzhen Agricultural Science and Technology Promotion Center, Shenzhen 518000, China

4. State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475004, China

5. Food Crops Research Institute, Yunnan Academy of Agricultural Sciences (YAAS), Yunnan Seed Laboratory, Yunnan Key Laboratory for Rice Genetic Improvement, Kunming 650200, China

Abstract

Bacterial blight is a destructive disease in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). Single resistance genes often have limitations in providing broad-spectrum resistance, as pathogens continuously evolve and vary. Breeding rice varieties with multiple disease resistance genes has proven to be an effective strategy for controlling bacterial blight. In this study, a single Cas9/gRNA construct was used to target the homologous sequences of Xa13 and Xa25 genes through destroying the target gene function, creating bacterial blight resistance in five rice varieties. These materials provide promising germplasm resources for the development of rice varieties with durable resistance to bacterial blight.

Funder

the ‘5511’ Collaborative Innovation Project of Fujian Academy of Agricultural Sciences

the Special Project for Public Welfare of the Research Institute of Fujian Province

Shenzhen Science and Technology Program

Publisher

MDPI AG

Link

https://www.mdpi.com/2073-4395/14/4/800/pdf

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