Biological Control Effect of Antagonistic Bacteria on Potato Black Scurf Disease Caused by Rhizoctonia solani

Author:

Lan Qingqing1,Liu Yang1,Mu Rongrong1,Wang Xuetao1,Zhou Qian1,Islam Rehmat2ORCID,Su Xu3,Tian Yongqiang1

Affiliation:

1. Laboratory of Tian Yongqiang Scientific Research 101, College of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China

2. Key Laboratory of Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an 710072, China

3. Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Tibet Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, China

Abstract

Potato black scurf, caused by Rhizoctonia solani, is a severe soil-borne disease that affects the quality and production of potatoes worldwide. In our study, we used Paenibacillus polymyxa YF and Bacillus amyloliquefaciens HT to determine the antagonistic ability of R. solani, with a particular focus on the antagonistic action of P. polymyxa YF to R. solani and its biocontrol effect on potato black scurf. In fermentation, filtrate assay, 50% filtrate of P. polymyxa YF and B. amyloliquefaciens HT inhibited the growth of R. solani by 85.55% and 82.86%, respectively. Microscopic observations showed notable morphological changes with mycelial collapse, atrophy, and deformation following treatment with the antagonistic filtrates. Moreover, cell membrane permeability results showed increased conductivity in bacteria-treated samples compared to the control. P. polymyxa YF exhibited stable colonization on potato plants and secreted various extracellular enzymes (protease, amylase, and cellulase), along with the synthetic substances with growth-promoting effects, such as siderophores and Indole-3-acetic acid (IAA). Whether it is in the excised tissue inoculation or potted experiment, the negative control showed the highest rank of disease symptoms. In the pot experiment, after YF treatment, physiological parameters showed remarkable changes in plant height, root length, stem thickness, and dry and fresh weight. Compared to blank control, the activities of the four resistant enzymes increased significantly in the P. polymyxa YF treatment group. The upregulation in the P. polymyxa YF group was 4.04, 0.54, 0.46, and 3.10 times, respectively. PCR analysis identified genes in both bacterial strains coding for antimicrobial lipopeptides, including fenB, ituC, and srfAA, which are associated with fengycin, iturin, and surfactin synthesis. These findings demonstrated that P. polymyxa YF has a prominent antagonistic effect on R. solani, suggesting its potential as an effective biological control agent for controlling potato black scurf.

Funder

Gansu Province Education Department

Qinghai Provincial Central Government Guide Local Science and Technology Development Project

Gansu Province Key Research and Development Project

Lanzhou Talent Innovation and Entrepreneurship Project

Gansu Jiayuguan Key Research and Development Project

Young People Fund of Lanzhou Jiaotong University

Publisher

MDPI AG

Reference65 articles.

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