Genome-Wide Identification, Characterization, and Expression Analysis of TUBBY Gene Family in Wheat (Triticum aestivum L.) under Biotic and Abiotic Stresses

Abstract

The TUBBY gene family is a group of transcription factors found in animals and plants with many functions. TLP genes have a significant role in response to different abiotic stresses. However, there is limited knowledge regarding the TUBBY gene family in T. aestivum. Here we identified 40 TaTLP genes in wheat to reveal their potential function. This study found that TUBBY (TaTLP) genes are highly conserved in wheat. The GO analysis of TaTLP genes revealed their role in growth and stress responses. Promoter analysis revealed that most TaTLPs participate in hormone and abiotic stress responses. The heatmap analysis also showed that TaTLP genes showed expression under various hormonal and abiotic stress conditions. Several genes were upregulated under different hormonal and temperature stresses. The qRT-PCR analysis confirmed our hypotheses. The results clearly indicate that various TaTLP genes showed high expression under temperature stress conditions. Furthermore, the results showed that TaTLP genes are expressed in multiple tissues with different expression patterns. For the first time in wheat, we present a comprehensive TaTLP analysis. These findings provide valuable clues for future research about the role of TLPs in the abiotic stress process in plants. Overall, the research outcomes can serve as a model for improving wheat quality through genetic engineering.