Nitrogen Application Alleviates Impairments for Jatropha curcas L. Seedling Growth under Salinity Stress by Regulating Photosynthesis and Antioxidant Enzyme Activity

Abstract

Jatropha curcas L. is a promising bioenergy source, and its seedling stage is sensitive to salinity. Nitrogen application presents an effective strategy for alleviating the adverse consequences of salinity stress. However, the responses of plant growth and physiology of Jatropha curcas L. seedlings to nitrogen application under salinity stress remain unclear. As a result, a one-year greenhouse plot experiment was conducted to investigate the effects of nitrogen application on the plant growth, antioxidant enzyme activity, and photosynthesis of Jatropha curcas L. seedlings under saline conditions. Experiment treatments consisted of three salinity stresses (mild salinity stress, S1: 2 g/kg; moderate salinity stress, S2: 4 g/kg; and severe salinity stress, S3: 6 g/kg), four nitrogen application rates (N0: 0 gN/plant; N1: 20 gN/plant; N2: 60 gN/plant; and N3: 100 gN/plant), and a control treatment (CK) which was without salinity stress and nitrogen application. The results showed that salinity stress substantially reduced plant growth of Jatropha curcas L. seedlings. As the salinity stress increased, the reduction in plant growth also increased. The S3 treatment had the lowest leaf area, leaf biomass, and total biomass, which decreased by an average of 70.4%, 66.3%, and 69.9%, respectively, compared to CK. Nitrogen application could compensate for these impairments of plant growth from salinity stress by promoting antioxidant enzyme activity and photosynthesis. As for mild and moderate salinity stresses, the maximum plant growth was found in the N3 treatment, with the maximum antioxidant enzyme activity, photosynthetic pigment, photosynthetic characteristic, and chlorophyll fluorescence. As for severe salinity stress, higher plant growth was found in N2 and N3 treatments, and there were no significant differences between N2 and N3 treatments. It also should be noted that the maximum photosynthetic characteristic and chlorophyll fluorescence were found in N2 treatment under severe salinity stress. In conclusion, nitrogen application could be an alternative strategy to improve the salinity tolerance of Jatropha curcas L. growth. The nitrogen application rate of 100 gN/plant could be recommended for low and moderate salinity stresses, while 60 gN/plant could be recommended for severe salinity stress. However, higher nitrogen application rate (>100 gN/plant) under mild and moderate salinity stress and the effects of reactive oxygen species under salinity stress should be further evaluated.