Screening, Identification, and Optimization of Fermentation Conditions of an Antagonistic Endophyte to Wheat Head Blight

Abstract

Fusarium Head Blight (FHB, scab) is a destructive fungal disease that causes extensive yield and quality losses in wheat and other small cereals. Biological control of FHB is considered to be an alternative disease management strategy that is environmentally benign, durable, and compatible with other control measures. In this study, to screen antagonistic bacteria with the potential to manage FHB, 113 endophytes were isolated from the stems, leaves, panicles, and roots of wheat. Among them, six strains appeared to effectively inhibit Fusarium graminearum growth and one isolate, XS-2, showed a highly antagonistic effect against FHB. An in vitro antagonistic test of XS-2 on wheat heads confirmed that XS-2 could suppress the disease severity of FHB. The 16S rDNA sequence analysis revealed that XS-2 is a strain of Bacillus amyloliquefaciens. Antagonistic spectrum analyses showed that XS-2 had antagonistic effects against two and four types of cotton and fruit tree pathogens, respectively. The fermentation condition assays showed that glucose and peptone are the most suitable nutrient sources for XS-2, and that the optimal pH value and temperature for fermentation were 7.4 and 28 °C, respectively. Our study indicates that XS-2 has a good antagonistic effect on FHB and lays a theoretical foundation for the application of the strain as a biological agent in the field to control FHB.