Effects of Italian Ryegrass (IRG) Supplementation on Animal Performance, Gut Microbial Compositions and Odor Emission from Manure in Growing Pigs

Abstract

Fermentable carbohydrate (FC) is a promising material to reduce odor emission from pig manure. This study was conducted to investigate the impact of diets containing Italian ryegrass (IRG), as a FC, on animal performance, odorous chemical and bacterial composition of manure. Pigs were weighed and fed diets containing various levels of IRG powder (0%, 0.5%, 1.0% and 1.5%) for 28 days. At the end of the trial, manure was collected to analyze the chemical composition, odorous compounds and bacterial community structure. As dietary IRG levels increased, concentrations of phenols and indoles were decreased by 12% and 37% compared with control, respectively, without changes in growth performance. IRG treatment increased the relative abundances of genera belong to the family Lachnospiraceae, Ruminococcaceae, Veillonellaceae, Peptostreptococcaceae and Lactobacillaceae, in order Clostridiales of phylum Firmicutes, but decreased the relative abundances of genus Sphaerochaeta in phylum Spirochaetes and genus AB243818_g of family Porphyromonadaceae in phylum Bacteroidetes when compared with control. Results from the current study demonstrate that IRG supplemented diets had a beneficial effect of reducing the odorous compounds in manure, possibly by altering the bacterial community structure towards predominantly carbohydrate utilizing microorganisms in the large intestine.