Qfhb.yzu.3B.1 and Qfhb.yzu.6B.3 Are Stable Quantitative Trait Loci for Wheat Resistance to Fusarium Head Blight with Diverse Genetic Backgrounds

Abstract

Fusarium head blight (FHB) can cause serious yield loss and significant mycotoxin contamination, which seriously threaten global food security and safety. Breeding stable and durable cultivars that are resistant to FHB is one of the most effective approaches to controlling this disease. Fhb1 is a well-known genetic locus for FHB resistance, but its resistance is not always effective across diverse wheat genetic backgrounds. To achieve a high and durable level of resistance, the discovery and use of additional quantitative trait loci (QTL) for FHB resistance are essentially needed in breeding programs. In this study, two independent wheat natural populations of different origins were used for mining resistance QTL with a major and stable effect. Using genome-wide association analysis (GWAS), a total of 58 marker–trait associations (MTAs) on chromosomes 1A, 2B, 3A, 3B, 4A, 4B, 4D, 5A, 5B, 5D, 6A, 6B, 6D, 7A and 7B were found to be significant for type II resistance to FHB. These 58 MTAs represent 24 putative QTL. Among these QTL, Qfhb.yzu.3B.1 and Qfhb.yzu.6B.3 were stably detected in the two natural populations across three consecutive experimental years. The favorable haplotypes at the two QTL could significantly reduce the disease severity, either individually or in combination. These two QTL are also additive to Fhb1 in cultivars with different genetic backgrounds. Breeder-friendly markers were designed to differentiate the contrasting alleles at these two loci, thus proving very useful for improving FHB resistance in wheat by marker-assisted selection.