Comparison of the Virulence of Space Mutants of Aspergillus oryzae XJ-1 against Adult Locusta migratoria-Reference-Cited by-同舟云学术

Comparison of the Virulence of Space Mutants of Aspergillus oryzae XJ-1 against Adult Locusta migratoria

Published:2024-01-02 Issue:1 Volume:14 Page:116
ISSN:2073-4395
Container-title:Agronomy
language:en
Short-container-title:Agronomy

Author:

Fu Xin¹²,Liu Hui¹³,Xu Xiao¹³,Guo Jin¹,Hu Shaojing¹,You Yinwei¹³,Zhang Long¹³

Affiliation:

1. Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, China

2. College of Grassland Science and Technology, China Agricultural University, Beĳing 100083, China

3. Shandong Provincial Engineering Technology Research Center on Bio-Control of Crops Diseases and Insect Pest, Jinan 250100, China

Abstract

Biological control methods provide a sustainable approach for reducing agricultural losses caused by locust plagues. Space mutagenesis can generate high numbers of mutations using satellites and spacecrafts, including beneficial and stable mutants. Aspergillus oryzae XJ-1 was recently reported to show high virulence against locusts. We subjected this fungal pathogen to space mutagenesis to obtain more effective strains. Pathogen conidia powder was mutated in the China Space Station for 6 months. We obtained five mutants of A. oryzae XJ-1, TQ201, TQ238, TQ302, TQ549, and TQ555, and all mutants were identified as A. oryzae by molecular techniques. TQ549 showed the highest virulence against adult L. migratoria (LT50: 4.97 ± 0.21 days); the LT50 of A. oryzae XJ-1 was 5.67 ± 0.06 days. Both TQ549 and A. oryzae XJ-1 grew most rapidly at 33 °C on potato dextrose agar (PDA) plates. There was no significant difference in the growth rate of TQ549 and A. oryzae XJ-1 at 24 °C. The colony morphological characteristics of the five mutants on PDA plates differed from that of A. oryzae XJ-1. The space mutant TQ549, which showed high virulence against adult locusts, could be used as a biological control agent for the control of locust infestations.

Funder

National Key R&D Program of China

Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences

Publisher

MDPI AG

Link

https://www.mdpi.com/2073-4395/14/1/116/pdf

Reference31 articles.

1. Locust and grasshopper management;Zhang;Annu. Rev. Entomol.,2019

2. Lecoq, M., and Zhang, L. (2019). Encyclopedia of Pest Orthoptera of the World, China Agricultural University Press.

3. Development of a desert locust plague;Bennett;Nature,1975

4. Biological control of locusts and grasshoppers;Lomer;Annu. Rev. Entomol.,2001

5. Kumar, J., Ramlal, A., Mallick, D., and Mishra, V. (2021). An overview of some biopesticides and their importance in plant protection for commercial acceptance. Plants, 10.