Genome-Wide Association Analysis of Cowpea Mild Mottle Virus Resistance in Soybean Germplasms from Northeast China
Author:
Luan Yameng1ORCID, Yang Siqi2, Wang Yuting1, Zhao Yu1, Wu Xiaoyun1, Chen Qingshan2, Qi Zhaoming2, Wu Xiaoxia3, Ji Weiqin1, Cheng Xiaofei1ORCID
Affiliation:
1. College of Plant Protection, Northeast Agricultural University, Harbin 150030, China 2. College of Agriculture, Northeast Agricultural University, Harbin 150030, China 3. College of Life Science, Northeast Agricultural University, Harbin 150030, China
Abstract
Cowpea mild mottle virus (CpMMV) is an important viral pathogen that seriously influences the yield and seed quality of soybeans worldwide. Resistance breeding is one of the most effective, economical, and environmentally safe strategies for controlling the disease caused by CpMMV. However, only few resistance genes have been identified in soybeans. In this study, the resistance of 169 soybean germplasms from Northeast China to a CpMMV strain isolated from soybean in China was evaluated, and a genome-wide association study (GWAS) was then performed to find possible resistance genes in these soybean germplasms. Nine resistant soybean germplasms were identified and two single nucleotide polymorphism sites (SNPs) were found to be closely associated with CpMMV resistance. A total number of 51 and 25 candidate genes neighboring the resistance-associated SNPs on chromosomes 6 and 12, respectively, were identified, among which one receptor-like kinase (RLK) on chromosome 6 and 2 toll-interleukin-1 receptor nucleotide-binding leucine-rich repeat receptors (TNLs) on chromosome 12 were recognized as the most probable resistance genes, respectively. Together, these data provide new insights on the resistance resources of soybeans to CpMMV, which will benefit the breeding of CpMMV-resistant soybean cultivars.
Funder
Natural Science Foundation of Heilongjiang Province of China Leading Talent Support Program of the Northeast Agricultural University
Reference48 articles.
1. GmMs1 encodes a kinesin-like protein essential for male fertility in soybean (Glycine max L.);Nadeem;J. Integr. Plant Biol.,2021 2. Hartman, G.L., Rupe, J.C., Sikora, E.J., Domier, L.L., Davis, J.A., and Steffey, K.L. (2015). Compendium of Soybean Diseases and Pests, APS Press. [5th ed.]. 3. Cowpea mild mottle, a newly recognized virus infecting cowpeas (Vigna unguiculata) in Ghana;Brunt;Ann. Appl. Biol.,1973 4. Almeida, A.M.R., Piuga, F.F., Kitajima, E.W., Gaspar, J.O., Valentin, N., Benato, L.C., Marin, S.R., Binneck, E., De Oliveira, T., and Belintani, P. (2003). Necrose da haste da soja. ProBiota Sér Doc., 221. Available online: https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPSO-2010/24143/1/doc221.pdf. 5. Molecular and biological characterization of cowpea mild mottle virus isolates infecting soybean in Brazil and evidence of recombination;Zanardo;Plant Pathol.,2014
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