Identification and Characterization of the BnFAR1/FHY3 Gene Family and Expression Analysis under Shading and Low-Temperature Responses in Brassica napus L.
Author:
Han Yuekun1, Cheng Hefen1, Jiang Yaolan1, Wang Xinwen1, Liu Xiaolan1, Zhang Dawei12, Wu Jinfeng12, Liu Lili12, Yan Mingli3, Zhou Dinggang12ORCID
Affiliation:
1. Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization, School of Life Science, Hunan University of Science and Technology, Xiangtan 411201, China 2. Key Laboratory of Ecological Remediation and Safe Utilization of Heavy Metal-Polluted Soils, College of Hunan Province, Xiangtan 411201, China 3. Crop Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410000, China
Abstract
FHY3 and FAR1 serve as positive regulators of the phyA-mediated far-red light signal pathway, influencing plant growth and development by regulating the expression of downstream genes. However, little is known about the FAR1/FHY3 family in Brassica species. A total of 21 members of the BnFAR1/FHY3 gene family were identified in the Brassica napus genome, exhibiting an uneven distribution across ten B. napus chromosomes. A phylogenetic analysis showed that the BnFAR1/FHY3 family could be divided into four subfamilies. Putative cis-elements in the BnFAR1/FHY3 promoter regions were also identified, which were potentially involved in phytohormone, light and abiotic stress responses (shading, low-temperature, etc.). Additionally, qRT-PCR results indicated that the expression levels of BnFAR1-10, BnFAR1-11, BnFAR1-21 and BnFAR1-4 decreased under shading stress. The expression of BnFAR1-10, BnFAR1-11 and BnFAR1-21 increased under low-temperature stress, whereas the expression of BnFAR1-4 did not change. In addition, the results of the tissue expression analysis showed that most of the genes exhibited the lowest expression in pollen and the highest expression in the 54-day silique. This study screened a batch of BnFHY3/BnFAR1 gene resources, which will contribute to further research on the functional characteristics of BnFHY3/BnFAR1 family members in growth, development and the stress response.
Funder
National Natural Science Foundation of China Funding of Hunan Provincial Department of Education College Student Innovation and Entrepreneurship Training Project in Hunan Province
Subject
Agronomy and Crop Science
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