Optimized Nitrogen Fertilization Promoted Soil Organic Carbon Accumulation by Increasing Microbial Necromass Carbon in Potato Continuous Cropping Field

Abstract

The form and distribution of organic carbon in soil affect its stability and storage, and nitrogen (N) fertilization can affect the transformation and accumulation of soil organic carbon (SOC), whereas how the N fertilizer rate affects SOC storage by regulating its fractions in a potato continuous cropping system is unknown. A 6-year field experiment was conducted to study the effect of different N fertilizer rates (NE (Nutrient Expert) –N, NE–1/2N, NE, and NE+1/2N) on the changes in SOC and its fractions in a potato continuous cropping system in North China. Soil NO3−-N gradually increased with increasing N fertilizer rates, whereas the N fertilizer rate had less effect on NH4+-N. Compared with the NE−N treatment, the increasing N fertilization increased the SOC and its components, whereas these C fractions did not continue to increase or began to decrease after N fertilization exceeded the rate applied in the NE treatment. While the increase in mineral-associated organic C (MAOC; 16.1–17.2% and 26.1–52.7% in the 0–20 cm and 20–40 cm layers, respectively) was greater than that of particulate organic C (POC; 3.7–7.4% and 11.5–16.4% in the 0–20 cm and 20–40 cm layers, respectively), the increase in bacterial necromass C (BNC; 9.2–21.8% and 28.9–40.4% in the 0–20 cm and 20–40 cm layers, respectively) was greater than that of fungal necromass C (FNC; 6.2–10.1% and 7.1–24.9% in the 0–20 cm and 20–40 cm layers, respectively). Furthermore, the increase in FNC was greater than that of BNC in the 20–40 cm layer of the same treatment. SOC was significantly and positively correlated with MAOC and FNC, and the correlation between SOC and both MNC and FNC was more significant in the 20–40 cm layer than in the 0–20 cm layer. Overall, in the potato continuous cropping system in North China, N fertilization improved SOC storage by increasing MNC to form MAOC, and optimizing N fertilization based on the NE system could better balance the increase and mineralization loss of SOC to achieve high SOC sequestration.