Effects of Ni and Cu Stresses on Morphological and Physiological Characteristics of Euphorbia marginata Pursh Seedlings

Abstract

Increasing soil contamination with nickel (Ni) and copper (Cu) is a growing environmental concern, adversely affecting ecosystems and the survival of both plants and animals. This study investigated the morphological and physiological responses of Euphorbia marginata Pursh seedlings to varying concentrations of Ni and Cu over a 45-day period. The findings revealed that low concentrations of Ni and Cu enhanced morphological indexes, root indexes, biomass, and photosynthetic pigment content of E. marginata, while high concentrations inhibited these parameters. Compared to the control, Ni and Cu stresses induced membrane peroxidation, increased cell membrane permeability, and inhibited the synthesis of soluble proteins and proline in the leaves. The seedlings demonstrated an ability to mitigate Ni and Cu toxicity by increasing soluble sugar content and enhancing the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Notably, E. marginata exhibited a higher capacity for Cu2+ enrichment and translocation compared to Ni2+. Combined Ni and Cu treatments reduced the maximum enrichment and translocation levels of both metals in E. marginata. This study highlights the superior tolerance of E. marginata to Ni and Cu stresses and elucidates the mechanisms underlying its response, providing a theoretical basis for the use of landscape plants in the remediation of heavy-metal-contaminated soils.