Transcriptomic Analysis of Sodium-Silicate-Induced Resistance against Rhizoctonia solani AG-3 in Potato

Author:

Feng Yayan1,Hao Jianjun2ORCID,Zhang Dongmei13,Huo Hongli13ORCID,Li Lele1,Xiu Zhijun13,Yang Chunfang13,Zhang Xiaoyu1ORCID

Affiliation:

1. Key Laboratory of Biopesticide Creation and Resource Utilization in Inner Mongolia Autonomous Region, College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China

2. School of Food and Agriculture, University of Maine, Orono, ME 04469, USA

3. Plant Protection Institute, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China

Abstract

Stem canker and black scurf of potatoes, caused by Rhizoctonia solani, are economically important diseases. Although the field application of sodium silicate has been shown to improve potato’s resistance against R. solani, the underlying mechanism remains unclear. In this study, we examined this resistance using transcriptomic analysis. Potato stems inoculated with R. solani were treated with sodium silicate, while a control group received no sodium silicate treatment. The plants were grown under natural environmental conditions at the farm of Inner Mongolia Agricultural University. Potato stems were sampled 4, 8, and 12 days after treatment. Total RNA was extracted using the TRIzol reagent and transformed into cDNA. The cDNA was sequenced, the reads were aligned, and the expression levels of genes were quantified and compared between the treated and control groups. A total of 1491 genes were identified as differentially expressed genes (DEGs). Furthermore, these DEGs were found to be involved in hydrolase activity, plant–pathogen interactions, hormone signal transduction, and the phenylpropanoid biosynthesis pathway. To confirm the up- and down-regulation of DEGs, quantitative real-time polymerase chain reaction (qRT-PCR) was performed on randomly selected genes. The results showed that the application of sodium silicate induces a complex defense network in potato plants involving physical barriers, innate immunity, phytohormone signaling, and various phenylpropanoid compounds to combat R. solani infection. This study provides valuable insights into the molecular mechanisms underlying sodium-silicate-induced resistance and its potential for reducing stem canker and black scurf in potato crops.

Funder

Inner Mongolia Science and Technology Project

Publisher

MDPI AG

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