Response of Annual Weeds to Glyphosate: Evaluation and Optimization of Application Rate Based on Fecundity-Avoidance Biomass Threshold Criterion

Abstract

The increased availability and high adoption rate of glyphosate-tolerant crops have selected for several glyphosate-resistant weed species. The response of representative weed species to glyphosate was assessed to provide insights and tools for optimizing glyphosate use for economic, agronomic and environmental reasons. Anoda cristata, Chenopodium album, Digitaria sanguinalis, Eleusine indica and Portulaca oleracea were grown outdoors in pots containing commercial potting medium. An increasing dose of glyphosate was applied to these species at three growth stages. Weed response was evaluated visually compared to the nontreated control and shoot dry weights were recorded. Fecundity was also determined. Based on visual evaluations, the dose of glyphosate required to attain 90% control of the species tested exhibited an application rate margin up to 28.5-fold compared to recommended rate, denoting a potential for rate optimization. Except for A. cristata, the recommended dose of glyphosate could be reduced by 30%–60% and still achieve 90% or greater control. The order of species sensitivity, based on effective dose 50 (ED50 )values, was E. indica > C. album > D. sanguinalis > P. oleracea > A. cristata. The ratio of ED90/ED50 was constant, indicating that increasing the glyphosate dose 8.7-fold would reduce weed biomass 1.8-fold. In most cases, the fecundity-avoidance biomass threshold (i.e., the maximum allowable weed biomass for herbicide application in order to prevent weed seed production and dispersal) for glyphosate was below the ED90 value. Complimentary measures such as fecundity-avoidance biomass threshold will improve herbicide evaluation procedures and preserve the effectiveness of herbicides, including glyphosate, on sensitive species, an important issue particularly when action to reduce herbicide resistance development is highly required.