The Agropyron mongolicum bHLH Gene AmbHLH148 Positively Involved in Transgenic Nicotiana benthamiana Adaptive Response to Drought Stress

Author:

Zhang Xuefeng1,Ma Yanhong1,Fan Bobo1,Sun Fengcheng2,Zhai Yongqing1,Zhao Yan3,Nie Lizhen2,Fang Yongyu2,Yu Zhuo1,Qi Bingjie1

Affiliation:

1. Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018, China

2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China

3. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010018, China

Abstract

While bHLH transcription factors have been linked to the regulation of various abiotic stressors, research on drought-related bHLH proteins and their molecular processes in Agropyron mongolicum has remained limited. In this study, a bHLH gene from A. mongolicum, designated as AmbHLH148, was successfully cloned and isolated. AmbHLH148 was exclusively localized within the nucleus. Additionally, qRT-PCR analysis demonstrated a significant upregulation of AmbHLH148 in response to drought stress. When transferred into tobacco (Nicotiana benthamiana), the heterologous expression of AmbHLH148 led to enhanced drought tolerance. Under drought stress conditions, AmbHLH148-OE transgenic tobacco plants exhibited increased activities of antioxidant defense enzymes, such as SOD (superoxide dismutase), POD (peroxidase), and CAT (catalase). These enzymes efficiently mitigated the accumulation of reactive oxygen species (ROS) compared to wild-type plants. Furthermore, AmbHLH148-OE transgenic tobacco showed elevated levels of PRO (proline) and reduced MDA (malondialdehyde) content, contributing to enhanced stability in the plant’s cell membrane system during drought stress. In summary, this study underscores that the overexpression of AmbHLH148 in transgenic tobacco acts as a positive regulator under drought stress by enhancing the plant’s antioxidant capacity. These findings shed light on the molecular mechanisms involved in bHLH transcription factors’ role in drought resistance, contributing to the discovery and utilization of drought-resistant genes in A. mongolicum for enhancing crop drought resistance.

Funder

National Natural Science Foundation of China

Publisher

MDPI AG

Subject

Agronomy and Crop Science

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