Comprehensive Control System for Ginger Bacterial Wilt Disease Based on Anaerobic Soil Disinfestation

Abstract

Bacterial wilt is a soil-borne disease that causes severe damage in ginger-growing regions of Japan (eight prefectures in the Shikoku, Kyushu, and Honshu regions). Because the pathogen Ralstonia pseudosolanacearum usually lives in deeper soil and infects host plants via the roots, it is not easy to eliminate even with chemical pesticides (such as soil fumigants). In our previous study, we found that anaerobic soil disinfestation with diluted ethanol (Et-ASD) effectively eliminated this pathogen. We conducted field experiments to confirm the effectiveness of Et-ASD in a ginger monoculture system. Eight trials were conducted in seven different ginger fields from spring to autumn. Excluding one trial in early spring, seven trials in summer successfully eliminated the pathogen from the field soil (below the detection limit by the developed sensitive bio-PCR method), and no disease recurrence was observed after ASD treatment. In addition, other useful methods for controlling the disease were explored, including proper field management after the disease outbreak and the disinfection of seed rhizomes. Based on these results, a comprehensive control system for bacterial wilt disease in ginger was developed.