An Evaluation of Bacterial Wilt (Ralstonia solanacearum) Resistance in a Set of Tomato Germplasm from the United States Department of Agriculture

Abstract

Bacterial wilt (BW), caused by Ralstonia solanacearum, is one of the devastating diseases in tomatoes (Solanum lycopersicum L.). The use of resistant cultivars and breeding for genetic resistance is the most effective, economical, and environmentally friendly management strategy for this disease. It is necessary to screen diverse germplasm and cultivated genotypes to identify resistant resources and to develop resistant cultivars in tomatoes to combat the changing pathogen isolates. This study evaluated 40 United States Department of Agriculture (USDA) tomato accessions for their BW resistance to the R. solanacearum isolate P822 under greenhouse conditions. The tomato plants were inoculated and visually assessed to observe their symptoms, and the disease severity was scored on a scale of 0 to 4 (0 = no leaf wilted, 1 = 25% of leaves wilted, 2 = 50% leaves wilted, 3 = 75% of leaves wilted, and 4 = 100% leaves wilted). Five accessions (PI 645370, PI 647306, PI 600993, PI 355110, and PI 270210) were observed as BW resistance, with PI 645370 showing the greatest resistance. The broad-sense heritability for BW resistance was estimated as 59.9% and 42.8% based on a 0–4 scale of disease incidence and the disease severity index, respectively. Two distinct clusters (sub-populations) were detected among 39 of the 40 accessions. The five identified BW-resistant accessions were distributed in both clusters, suggesting a likely difference in the genetic base among the five resistance accessions. The resistant accessions will contribute significantly to the tomato breeding program to develop new cultivars with BW resistance.