Effect of Melatonin on the Growth of Dendrobium officinale Protocorm-Like Bodies

Author:

Tang Jiaqi1,Huang Ruyan1,Yuan Dan1,Sun Han1,Li Yuping1,Pei Zekang1,Wang Congqiao1,Li Chengyue1,Qiu Dongliang1ORCID,Chen Qingqing1

Affiliation:

1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Abstract

Dendrobium officinale Kimura & Migo is a perennial herbaceous plant of the genus Dendrobium in the family of Orchidaceae with high medicinal value. Melatonin (MT) is an indole-like tryptamine with functions such as regulating plant growth and development. This experiment investigated the effects of different concentrations of MT on the growth and development of protocorms of D. officinale protocorm-like bodies (PLBs). The results showed that the changes in morphological indicators such as color, cluster size, and surface changes were more significant under 75 µM MT than those of 0 µM (CK), and the appearance of white on the PLB surface was expedited, which was more conducive to the proliferation of PLBs. MT treatment of 100 µM inhibited the differentiation of adventitious buds, and the contents of photosynthetic pigments, polysaccharides, and flavonoids were significantly increased. Moreover, as compared with CK, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in PLBs increased significantly, while the content of malondialdehyde (MDA) decreased gradually with 75 µM or less. In conclusion, a concentration of 75 µM melatonin can enhance the rapid propagation rate of D. officinale in vitro, providing insights into the effects of melatonin on the growth of tissue-cultured D. officinale seedlings.

Funder

Fujian Provincial Education Department

Publisher

MDPI AG

Reference65 articles.

1. In vitro propagation by asymbiotic seed germination and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity studies of tissue culture raised plants of three medicinally important species of dendrobium;Lo;Biol. Pharm. Bull.,2004

2. Symbiotic in vitro seed propagation of Dendrobium: Fungal and bacterial partners and their influence on plant growth and development;Silva;Planta,2015

3. Structure characterization and immunomodulating effects of polysaccharides isolated from Dendrobium officinale;Wei;Planta Medica,2016

4. Chemical properties and antioxidant activity of a water-soluble polysaccharide from Dendrobium officinale;Luo;Int. J. Biol. Macromol.,2016

5. Micropropagation of the endangered medicinal orchid, Dendrobium officinale;Chen;Life Sci. J.,2014

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