Transcriptome-Based Weighted Correlation Network Analysis of Maize Leaf Angle Regulation by Exogenous Brassinosteroid

Abstract

Maize (Zea mays L.) leaf angle is an important characteristic affecting high-density planting, and it is also a central indicator for maize plant type selection to improve yield. Brassinosteroids (BRs) are a class of phytohormones that could modulate the growth and development of plant leaf angles. However, its functional mechanism remains unclear in maize. In this study, we used maize self-line B73 as material to analyze the transcriptome of leaf cushion after BR treatment at the seedling stage. Using seven concentrations of exogenous BR-treated maize B73 plants, the results show that the leaf angle and the cell length near the leaf pillow increased and then decreased with BR concentration increasing, and the 50 μM level was the best treatment. Analysis of 11,487 differences expressed genes (DEGs) found that genes related to cell volume were up-regulated, and the expression of genes related to the cell division was down-regulated. It is speculated that exogenous BR regulates the size of the maize leaf angle by regulating cell volume and cell division, and so we constructed a molecular mechanism model of maize response to exogenous BR. The molecular mechanism model of exogenous BR through weighted gene co-expression network analysis (WGCNA) DEGs, and two gene modules related to changes in maize leaf angle were identified. The results can provide a theoretical basis for determining the mechanism of exogenous BR-regulated maize.