Quantitative Trait Locus Analysis of Microscopic Phenotypic Characteristic Data Obtained Using Optical Coherence Tomography Imaging of Rice Bacterial Leaf Blight Infection in the Field

Abstract

Rapid climate change has increased the incidence of various pests and diseases, and these threaten global food security. In particular, BLB (bacterial leaf blight) is caused by Xoo (Xanthomonas oryzae pv. oryzae) and its main characteristic is that the rice suddenly dries and withers. Recently, omics have been effectively used in agriculture. In particular, it is a key technology that can accurately diagnose diseases in the field. Until now, QTL (quantitative trait loci) mapping has been analyzed using only subjective phenotypic data by experts. However, in this study, diseases were accurately diagnosed using OCT (optical coherence tomography), and QTL mapping was performed using leaf thickness and leaf angles after Xoo inoculation. After Xoo inoculation of a 120 Cheongcheong/Nagdong double haploid (CNDH) population, QTL mapping was performed using the changing leaf angle, and OsWRKY34q1 was detected in RM811-RM14323 of chromosome 1. OsWRKY34q1 always had a higher expression level in the BLB-resistant population than in the susceptible population after Xoo inoculation. OsWRKY34q1 belongs to the WRKY family of genes. OsWRKY34q1 could be effectively used to develop BLB-resistant rice varieties in response to the current era of unpredictable climate change.