Alleviating Effect of Lipid Phytochemicals in Seed Oil (Brassica napus L.) on Oxidative Stress Injury Induced by H2O2 in HepG2 Cells via Keap1/Nrf2/ARE Signaling Pathway
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Published:2024-08-23
Issue:17
Volume:16
Page:2820
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ISSN:2072-6643
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Container-title:Nutrients
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language:en
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Short-container-title:Nutrients
Author:
Peng Simin12ORCID, Liao Luyan2ORCID, Deng Huiqing2, Liu Xudong3ORCID, Lin Qian14, Wu Weiguo2
Affiliation:
1. Hunan Provincial Key Laboratory of the Traditional Chinese Medicine Agricultural Biogenomics, Changsha Medical University, Changsha 410219, China 2. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China 3. State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410018, China 4. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410125, China
Abstract
α-tocopherol (α-T), β-sitosterol (β-S), canolol (CA), and sinapic acid (SA) are the four main endogenous lipid phytochemicals (LP) found in Brassica napus L. seed oil, which possess the bioactivity to prevent the risk of several chronic diseases via antioxidant-associated mechanisms. Discovering the enhancer effects or synergies between LP is valuable for resisting oxidative stress and improving health benefits. The objectives of this study were to identify a potentially efficacious LP combination by central composite design (CCD) and cellular antioxidant activity (CAA) and to investigate its protective effect and potential mechanisms against H2O2-induced oxidative damage in HepG2 cells. Our results indicated that the optimal concentration of LP combination was α-T 10 μM, β-S 20 μM, SA 125 μM, and CA 125 μM, respectively, and its CAA value at the optimal condition was 10.782 μmol QE/100 g. At this concentration, LP combination exerted a greater amelioration effect on H2O2-induced HepG2 cell injury than either antioxidant (tea polyphenols or magnolol) alone. LP combination could reduce the cell apoptosis rate induced by H2O2, lowered to 10.06%, and could alleviate the degree of oxidative damage to cells (ROS↓), lipids (MDA↓), proteins (PC↓), and DNA (8-OHdG↓). Additionally, LP combination enhanced the antioxidant enzyme activities (SOD, CAT, GPX, and HO-1), as well as the T-AOC, and increased the GSH level in HepG2 cells. Furthermore, LP combination markedly upregulated the expression of Nrf2 and its associated antioxidant proteins. It also increased the expression levels of Nrf2 downstream antioxidant target gene (HO-1, SOD-1, MnSOD, CAT, GPX-1, and GPX-4) and downregulated the mRNA expression levels of Keap1. The oxidative-stress-induced formation of the Keap1/Nrf2 complex in the cytoplasm was significantly blocked by LP treatment. These results indicate that LP combination protected HepG2 cells from oxidative stress through a mechanism involving the activation of the Keap1/Nrf2/ARE signaling pathways.
Funder
Scientific Research Innovation Program of Hunan Science and Technology Innovation Program of Hunan Province Hunan Engineering Technology Research Center for Rapeseed Oil Nutrition Health and Deep Development Oilseed Industry Technology System Processing Post expert project of Hunan Provincial Department of Agriculture and Rural Affairs Document
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