Yarrowia lipolytica as a Platform for Punicic Acid Production
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Published:2023-05-16
Issue:10
Volume:24
Page:8823
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Urbanikova Veronika1, Park Young-Kyoung2ORCID, Krajciova Daniela3, Tachekort Mehdi24, Certik Milan1ORCID, Grigoras Ioana4ORCID, Holic Roman3ORCID, Nicaud Jean-Marc2ORCID, Gajdos Peter1ORCID
Affiliation:
1. Institute of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology, 81237 Bratislava, Slovakia 2. Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy-en-Josas, France 3. Institute of Animal Biochemistry and Genetics, Centre of Biosciences, Slovak Academy of Sciences, 84005 Bratislava, Slovakia 4. Université Paris-Saclay, Univ Evry, CNRS, CEA, Génomique métabolique, 91057 Evry-Courcouronnes, France
Abstract
Punicic acid (PuA) is a polyunsaturated fatty acid with significant medical, biological, and nutraceutical properties. The primary source of punicic acid is the pomegranate seed oil obtained from fruits of trees that are mainly cultivated in subtropical and tropical climates. To establish sustainable production of PuA, various recombinant microorganisms and plants have been explored as platforms with limited efficiencies. In this study, the oleaginous yeast Yarrowia lipolytica was employed as a host for PuA production. First, growth and lipid accumulation of Y. lipolytica were evaluated in medium supplemented with pomegranate seed oil, resulting in the accumulation of lipids up to 31.2%, consisting of 22% PuA esterified in the fraction of glycerolipids. In addition, lipid-engineered Y. lipolytica strains, transformed with the bifunctional fatty acid conjugase/desaturase from Punica granatum (PgFADX), showed the ability to accumulate PuA de novo. PuA was detected in both polar and neutral lipid fractions, especially in phosphatidylcholine and triacylglycerols. Promoter optimization for PgFADX expression resulted in improved accumulation of PuA from 0.9 to 1.8 mg/g of dry cell weight. The best-producing strain expressing PgFADX under the control of a strong erythritol-inducible promoter produced 36.6 mg/L PuA. These results demonstrate that the yeast Y. lipolytica is a promising host for PuA production.
Funder
INRAE, the French National Research Institute for Agriculture, Food and Environment Micalis Institute Slovak Research and Development Agency Ministry of Education, Science, Research, and Sport of the Slovak Republic Slovak Academy of Sciences Genopole, University of Evry-val-d’Essonne University of Paris-Saclay, Investissements d’Avenir New England BioLabs Integrated DNA Technologies Inc. Twist Biosciences SnapGene Macherey Nagel Zymo Research Promega
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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