Step-by-Step Regeneration of Tentacles after Injury in Anemonia viridis—Morphological and Structural Cell Analyses
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Published:2023-05-16
Issue:10
Volume:24
Page:8860
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
La Corte Claudia1ORCID, Baranzini Nicolò2ORCID, Dara Mariano1ORCID, Bon Camilla2, Grimaldi Annalisa2ORCID, Parisi Maria Giovanna1ORCID, Zizzo Maria Grazia34ORCID, Cammarata Matteo1ORCID
Affiliation:
1. Marine Immunobiology Laboratory, Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy 2. Department of Biotechnology and Life Science, University of Insubria, Via Dunant 3, 21100 Varese, Italy 3. Department of Chemical and Pharmaceutical Biological Sciences and Technologies (STEBICEF), University of Palermo, Viale delle Scienze, Ed. 16, 90128 Palermo, Italy 4. Advanced Technologies Network (ATeN) Center, Università di Palermo, Viale delle Scienze, 90128 Palermo, Italy
Abstract
Benthic marine invertebrates, such as corals, are often subjected to injury caused by several sources. Here, the differences and characteristics in injured and health tissues in terms of cellular components are shown through a histological investigation of the soft coral Anemonia viridis at 0 h, 6 h, 24 h, and 7 days after injury caused by tentacle amputation. In addition, a new tool was used for the first time in invertebrates, positron emission tomography, in order to investigate the events that occur during regeneration within a longer time period (0 h, 24 h, and 14 days after the tentacles were cut). Higher integrated density values were measured through a densitometric analysis in sections stained with Fontana–Masson at 24 h after the tentacles were cut. This suggests an increase in melanin-like containing cells and a subsequent increase in fibroblast-like cells differentiated by amoebocytes that converge to the lesion site in the early stages of inflammation and regeneration. This work provides, for the first time, an elucidation of the events that occur during wound-healing and regeneration in basal metazoan, focusing on the characterisation of immune cells and their role. Our results indicate that Mediterranean anthozoan proves to be a valuable model for studying regeneration. Many events highlighted in this research occur in different phyla, suggesting that they are highly conserved.
Funder
Insubria Academic Research Funds Ministero dell’Istruzione, dell’Università e della Ricerca
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference40 articles.
1. Towards a Local Mass Mortality of the Mediterranean Orange Coral Astroides calycularis (Pallas, 1766) in the Pelagie Islands Marine Protected Area (Italy);Bisanti;Aquat. Conserv. Mar. Freshw. Ecosyst.,2022 2. Boström-Einarsson, L., Babcock, R.C., Bayraktarov, E., Ceccarelli, D., Cook, N., Ferse, S.C.A., Hancock, B., Harrison, P., Hein, M., and Shaver, E. (2020). Coral Restoration—A Systematic Review of Current Methods, Successes, Failures and Future Directions. PLoS ONE, 15. 3. Raymundo, L.J., Licuanan, W.L., and Kerr, A.M. (2018). Adding Insult to Injury: Ship Groundings Are Associated with Coral Disease in a Pristine Reef. PLoS ONE, 13. 4. The Nature of Tissue Regeneration after Wounding in the Sea Anemone Calliactis parasitica (Couch);Young;J. Mar. Biol. Assoc.,1974 5. Parisi, M.G., Grimaldi, A., Baranzini, N., La Corte, C., Dara, M., Parrinello, D., and Cammarata, M. (2021). Mesoglea Extracellular Matrix Reorganization during Regenerative Process in Anemonia viridis (Forskål, 1775). Int. J. Mol. Sci., 22.
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