Dazl and dnd Identify Both Embryonic and Gonadal Germ Cells in Chinese Hook Snout Carp (Opsariichthys bidens)

Author:

Yin Zifeng1,Xue Lingzhan2,Song Peng1,Jawad Muhammad1,Xu Cong1,Bu Weishao3,Li Mingyou1ORCID

Affiliation:

1. Key Laboratory of Integrated Rice-Fish Farming, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China

2. Freshwater Fisheries Research Institute of Fujian Province, Fuzhou 350002, China

3. Yunhe County Qingjiang Ecological Turtle Breeding Professional Cooperative, Lishui 323600, China

Abstract

In recent years, the Chinese hook snout carp (Opsariichthys bidens) is becoming increasingly popular due to its high nutritional value and delicious taste. However, anthropogenic influences have caused a rapid reduction in its population. Considering long-term development sustainability, it is essential to conduct research on its reproductive development. In this study, the germ-cell related genes dazl (Obdazl) and dnd (Obdnd) were cloned, and their expression patterns were revealed. RT-PCR analysis indicated that Obdazl and Obdnd were specifically expressed in gonads. In the testis, Obdazl and Obdnd RNAs have a similar expression pattern: their transcripts were abundant in spermatogonia, gradually decreased, and eventually disappeared during spermatogenesis. Early oocytes in the ovary exhibited strong expression of Obdazl and Obdnd RNAs and reduced remarkably after peak expression at III oocytes. Surprisingly, Obdazl RNA was dynamically found in perinuclear spots that finally aggregated into the Balbiani body (Bb) in the early oocytes. Moreover, Obdazl 3′ UTR enabled the reporter gene to be expressed stably in medaka PGCs, suggesting that dazl was identified as the germ cell marker in O. bidens, which may provide perspectives for further studies on the development of PGCs in the future.

Funder

National Natural Science Foundation of China

Fujian Provincial Department of Ocean and Fisheries Special Fund Project

The Seed Industry Innovation and Industrialization Project of Fujian Province

Publisher

MDPI AG

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3