Affiliation:
1. Agricultural Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Hat Yai 90110, Songkhla, Thailand
Abstract
The rubber tree is an important economic tree in Thailand. Recently, the cultivation of rubber trees in Thailand has suffered from a novel leaf fall disease with diverse symptoms, including leaf spot and leaf blight, resulting in severe leaf defoliation. Fungi from the Lasiodiplodia genus, which causes leaf disease in rubber trees, have not been reported in Thailand. Our research aimed to identify Lasiodiplodia associated with leaf blight disease in Thailand by examining morphological characteristics and completing a multi-gene sequence analysis and pathogenicity test to fulfill Koch’s postulates. The internal transcribed spacer regions, translation elongation factor 1-α, and β tubulin 2 were sequenced for the multi-gene sequence analysis. In total, we recovered 14 isolates with 6 of those isolates. Of the six pathogenetic isolates, LST001, LST002, LYT003, LSrt001, and LSrt002 were determined to be Lasiodiplodia chonburiensis, and isolate LYL005 was determined to be L. theobromae. To the best of our knowledge, this is the first report of L. chonburiensis and L. theobromae being associated with leaf blight disease in rubber trees in Thailand or elsewhere.
Funder
National Science, Research and Innovation Fund
Subject
Nature and Landscape Conservation,Agricultural and Biological Sciences (miscellaneous),Ecological Modeling,Ecology
Reference51 articles.
1. Characterization of Pestalotiopsis microspora, the causal agent of rubber leaf blight disease in Cameroon;Owona;Rubber Sci.,2018
2. Diversity of fungal endophytes in leaves and stems of wild rubber trees (Hevea brasiliensis) in Peru;Gazis;Fungal Ecol.,2010
3. Fernando, S., Silva, K., Tennakoon, I., Siriwardena, D., Wijayaratne, C., and Nishantha, N. (2020). Re-Emergence of the Abnormal Leaf Fall Disease Condition in Sri Lankan Rubber.
4. Identification and characterization of Neopestalotiopsis fungi associated with a novel leaf fall disease of rubber trees (Hevea brasiliensis) in Thailand;Pornsuriya;J. Phytopathol.,2020
5. Lasiodiplodia theobromae as a causal pathogen of leaf blight, stem canker, and pod rot of Theobroma cacao in Malaysia;Zakaria;Sci. Rep.,2022
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献