Passive Immunotherapy of Cynomolgus Monkeys with Anti-Rotavirus IgY

Author:

Bentes Gentil Arthur1ORCID,Guimarães Juliana Rodrigues1,Volotão Eduardo de Mello2,Lanzarini Natália Maria12ORCID,da Silva Alexandre dos Santos1,Gardinali Noemi Rovaris1,Marchevsky Renato Sergio3,Leite José Paulo Gagliardi2,de Oliveira Jaqueline Mendes1ORCID,Pinto Marcelo Alves1ORCID

Affiliation:

1. Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil

2. Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil

3. Laboratório de Ensaios Pré-Clínicos, Instituto de Tecnologia em Imunobiológicos, Fiocruz, Rio de Janeiro 21040-360, RJ, Brazil

Abstract

Immunoglobulins Y (IgY) purified from egg yolks of hens represents an attractive, cost-effective alternative for the development of new diagnostic and therapeutic platforms. In this study, we evaluated the therapeutic efficacy of rotavirus-specific IgY in a cynomolgus monkey (Macaca fascicularis) model. Animals were experimentally infected with human rotavirus Group A (RVA), the most common cause of severe acute diarrhoea among young children worldwide. Animals were administered human RVA (3.1 × 107 FFU/mL) by oral gavage, challenged with 2.5 mg of anti-RVA IgY orally, and monitored for five days according to clinical, haematological and biochemical parameters; serum electrolyte levels; viral shedding; and histopathological changes. Immunotherapy with anti-RVA IgY had a protective effect against severe rotavirus-induced enteritis in four of the ten treated monkeys, as evidenced by histopathological findings. Although only one animal had diarrhoea, all but one exhibited virus shedding regardless of the treatment.

Funder

National Council for Scientific and Technological Development

Carlos Chagas Filho Foundation for Research Support of Rio de Janeiro State

Oswaldo Cruz Institute

Publisher

MDPI AG

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