LC-AMP-F1 Derived from the Venom of the Wolf Spider Lycosa coelestis, Exhibits Antimicrobial and Antibiofilm Activities
-
Published:2024-01-19
Issue:1
Volume:16
Page:129
-
ISSN:1999-4923
-
Container-title:Pharmaceutics
-
language:en
-
Short-container-title:Pharmaceutics
Author:
Song Yuxin1, Wang Junyao1, Liu Xi1, Yu Shengwei1, Tang Xing2, Tan Huaxin1
Affiliation:
1. Institute of Biochemistry and Molecular Biology, Hengyang Medical College, University of South China, Hengyang 421001, China 2. Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region, College of Life Sciences, Hengyang Normal University, Hengyang 421002, China
Abstract
In recent years, there has been a growing interest in antimicrobial peptides as innovative antimicrobial agents for combating drug-resistant bacterial infections, particularly in the fields of biofilm control and eradication. In the present study, a novel cationic antimicrobial peptide, named LC-AMP-F1, was derived from the cDNA library of the Lycosa coelestis venom gland. The sequence, physicochemical properties and secondary structure of LC-AMP-F1 were predicted and studied. LC-AMP-F1 was tested for stability, cytotoxicity, drug resistance, antibacterial activity, and antibiofilm activity in vitro compared with melittin, a well-studied antimicrobial peptide. The findings indicated that LC-AMP-F1 exhibited inhibitory effects on the growth of various bacteria, including five strains of multidrug-resistant bacteria commonly found in clinical settings. Additionally, LC-AMP-F1 demonstrated effective inhibition of biofilm formation and disruption of mature biofilms. Furthermore, LC-AMP-F1 exhibited favorable stability, minimal hemolytic activity, and low toxicity towards different types of eukaryotic cells. Also, it was found that the combination of LC-AMP-F1 with conventional antibiotics exhibited either synergistic or additive therapeutic benefits. Concerning the antibacterial mechanism, scanning electron microscopy and SYTOX Green staining results showed that LC-AMP-F1 increased cell membrane permeability and swiftly disrupted bacterial cell membranes to exert its antibacterial effects. In summary, the findings and studies facilitated the development and clinical application of novel antimicrobial agents.
Funder
Scientific research project of Hunan Provincial Department of Education Hunan Graduate Research Innovation Project National Natural Science Foundation of China Hunan Provincial Natural Science Foundation Open Fund project of Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region
Subject
Pharmaceutical Science
Reference43 articles.
1. Ugwuanyi, F.C., Ajayi, A., Ojo, D.A., Adeleye, A.I., and Smith, S.I. (2021). Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Centerin Nigeria. Ann. Clin. Microbiol. Antimicrob., 20. 2. Tan, H., Wang, J., Song, Y., Liu, S., Lu, Z., Luo, H., and Tang, X. (2022). Antibacterial Potential Analysis of Novel α-Helix Peptides in the Chinese Wolf Spider Lycosa sinensis. Pharmaceutics, 14. 3. Elnagdy, S., Raptopoulos, M., Kormas, I., Pedercini, A., and Wolff, L.F. (2021). Local Oral Delivery Agents with Anti-Biofilm Properties for the Treatment of Periodontitis and Peri-Implantitis. A Narrative Review. Molecules, 26. 4. Vestby, L.K., Gronseth, T., Simm, R., and Nesse, L.L. (2020). Bacterial Biofilm and its Role in the Pathogenesis of Disease. Antibiotics, 9. 5. The importance of intracellular bacterial biofilm in infectious diseases;Mirzaei;Microb. Pathog.,2020
|
|