Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9-Reference-Cited by-同舟云学术

Advances in Nanoparticles as Non-Viral Vectors for Efficient Delivery of CRISPR/Cas9

Published:2024-09-11 Issue:9 Volume:16 Page:1197
ISSN:1999-4923
Container-title:Pharmaceutics
language:en
Short-container-title:Pharmaceutics

Author:

Kim Minse¹²,Hwang Youngwoo²³^ORCID,Lim Seongyu¹²,Jang Hyeon-Ki¹²⁴^ORCID,Kim Hyun-Ouk¹²^ORCID

Affiliation:

1. Division of Chemical Engineering and Bioengineering, College of Art, Culture and Engineering, Kangwon National University, Chuncheon 24341, Republic of Korea

2. Department of Smart Health Science and Technology, Kangwon National University, Chuncheon 24341, Republic of Korea

3. Department of Systems Immunology, Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea

4. Multidimensional Genomics Research Center, Kangwon National University, Chuncheon 24341, Republic of Korea

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system is a gene-editing technology. Nanoparticle delivery systems have attracted attention because of the limitations of conventional viral vectors. In this review, we assess the efficiency of various nanoparticles, including lipid-based, polymer-based, inorganic, and extracellular vesicle-based systems, as non-viral vectors for CRISPR/Cas9 delivery. We discuss their advantages, limitations, and current challenges. By summarizing recent advancements and highlighting key strategies, this review aims to provide a comprehensive overview of the role of non-viral delivery systems in advancing CRISPR/Cas9 technology for clinical applications and gene therapy.

Funder

National Research Foundation of Korea

Ministry of Science and ICT

Global-Learning & Academic research institution

Postdocs (LAMP) Program of the National Research Foundation of Korea

Publisher

MDPI AG

Link

https://www.mdpi.com/1999-4923/16/9/1197/pdf

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