Affiliation:
1. Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia
2. Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe 350-0815, Saitama, Japan
Abstract
Background: Electrospun fibers are widely studied in regenerative medicine for their ability to mimic the extracellular matrix (ECM) and provide mechanical support. In vitro studies indicated that cell adhesion and migration is superior on smooth poly(L-lactic acid) (PLLA) electrospun scaffolds and porous scaffolds once biofunctionalized with collagen. Methods: The in vivo performance of PLLA scaffolds with modified topology and collagen biofunctionalization in full-thickness mouse wounds was assessed by cellular infiltration, wound closure and re-epithelialization and ECM deposition. Results: Early indications suggested unmodified, smooth PLLA scaffolds perform poorly, with limited cellular infiltration and matrix deposition around the scaffold, the largest wound area, a significantly larger panniculus gape, and lowest re-epithelialization; however, by day 14, no significant differences were observed. Collagen biofunctionalization may improve healing, as collagen-functionalized smooth scaffolds were smallest overall, and collagen-functionalized porous scaffolds were smaller than non-functionalized porous scaffolds; the highest re-epithelialization was observed in wounds treated with collagen-functionalized scaffolds. Conclusion: Our results suggest that limited incorporation of smooth PLLA scaffolds into the healing wound occurs, and that altering surface topology, particularly by utilizing collagen biofunctionalization, may improve healing. The differing performance of the unmodified scaffolds in the in vitro versus in vivo studies demonstrates the importance of preclinical testing.
Reference44 articles.
1. (2022, November 23). Available online: https://www.globenewswire.com/news-release/2020/03/04/1995036/0/en/Global-Skin-and-Wound-Care-Market-Is-Expected-to-Reach-USD-25-98-Billion-by-2025-Fior-Markets.html.
2. Advances in skin regeneration: Application of electrospun scaffolds;Norouzi;Adv. Healthc. Mater.,2015
3. A comprehensive review of advanced biopolymeric wound healing systems;Mayet;J. Pharm. Sci.,2014
4. Azimi, B., Maleki, H., Zavagna, L., De la Ossa, J.G., Linari, S., Lazzeri, A., and Danti, S. (2020). Bio-based electrospun fibers for wound healing. J. Funct. Biomater., 11.
5. Biomimetic nanocomposite scaffolds based on surface modified PCLnanofibers containing curcumin embedded in chitosan/gelatin for skin regeneration;Ghaee;Compos. Part B,2019
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