Antimicrobial and Antiproliferative Activity of Green Synthesized Silver Nanoparticles Using Bee Bread Extracts
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Published:2023-06-23
Issue:7
Volume:15
Page:1797
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ISSN:1999-4923
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Container-title:Pharmaceutics
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language:en
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Short-container-title:Pharmaceutics
Author:
Urcan Adriana Cristina1ORCID, Criste Adriana Dalila1ORCID, Szanto Karina Ioana1, Ștefan Razvan2ORCID, Zahan Marius1ORCID, Muscă Adriana Sebastiana1, Focsan Monica3ORCID, Burtescu Ramona Flavia4, Olah Neli Kinga45ORCID
Affiliation:
1. Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 3-5 Mănăştur Street, 400372 Cluj-Napoca, Romania 2. Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania 3. Nanobiophotonics and Laser Microspectroscopy Center, Interdisciplinary Research Institute in Bio-Nano-Sciences, Babes-Bolyai University, Treboniu Laurian No. 42, 400271 Cluj-Napoca, Romania 4. PlantExtrakt Ltd., Rădaia, 407059 Cluj-Napoca, Romania 5. Faculty of Pharmacy, “Vasile Goldiş” Western University of Arad, 310414 Arad, Romania
Abstract
Bee bread (BB) is a fermented mixture of bee pollen, is rich in proteins, amino acids, fatty acids, polyphenols, flavonoids, as well as other bioactive compounds, and is considered functional food for humans. In this study, we explored an innovative green synthesis of colloidal silver nanoparticles, using BB extracts as reducing and stabilizing agents. A preliminary chemical characterization of the BB extracts was conducted. The plasmonic response of the as-synthesized silver nanoparticles (BB-AgNPs) was evaluated by UV–Vis spectroscopy, while their hydrodynamic diameter and zeta potential were investigated by dynamic light spectroscopy (DLS). Transmission electron microscopy (TEM) analysis pointed out polydisperse NPs with quasi-spherical shapes. The newly synthesized nanoparticles showed good antioxidant activity against the tested free radicals, DPPH, ABTS•+, and FRAP, the best results being obtained in the case of ABTS•+. BB-AgNPs exhibited good antibacterial activity on the tested Gram-positive and Gram-negative bacterial strains: herein S. aureus, B. cereus, E. faecalis, E. coli, P. aeruginosa, S. enteritidis, and on yeast C. albicans, respectively. The inhibition diameters varied between 7.67 ± 0.59 and 22.21 ± 1.06 mm, while the values obtained for minimum inhibitory concentration varied between 0.39 and 6.25 µg/mL. In vitro antiproliferative activity was tested on colon adenocarcinoma, ATCC HTB-37 cell line, and the results have shown that the green synthetized BB-AgNPs induced a substantial decrease in tumor cell viability in a dose-dependent manner with an IC50 ranging from 24.58 to 67.91 µg/mL. Consequently, more investigation is required to comprehend the processes of the cytotoxicity of AgNPs and develop strategies to mitigate their potentially harmful effects while harnessing their antimicrobial properties.
Funder
University of Agricultural Science and Veterinary Medicine Cluj-Napoca
Subject
Pharmaceutical Science
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