Affiliation:
1. Aquatic Disease Control Division, National Fisheries Products Quality Management Service, 216 Gijanghaean-ro, Gijang-eup, Gijang-gun, Busan 46083, Republic of Korea
Abstract
This study aimed to investigate the effects of different injection sites, including dorsal, cheek, and pectoral fin muscles, on the pharmacological properties of amoxicillin (AMOX) in olive flounder (Paralichthys olivaceus) after a single intramuscular (IM) injection of 40 mg/kg. The AMOX concentration was measured using high-performance liquid chromatography-tandem mass spectrometry, followed by a non-compartmental model analysis. The peak serum concentrations (Cmax) achieved 3 h after dorsal, cheek, and pectoral fin IM injections were 202.79, 203.96, and 229.59 μg/mL, respectively. The area under the concentration-time curve (AUC) was 1697.23, 2006.71, and 1846.61 µg/mL·h, respectively. The terminal half-life (t1/2λZ) was prolonged for cheek and pectoral fin IM injections (10.12 and 10.33 h, respectively) compared to dorsal IM injection (8.89 h). In the pharmacokinetic-pharmacodynamic analysis, a higher T > minimum inhibitory concentration (MIC) and AUC/MIC values were observed after AMOX was injected into the cheek and pectoral fin muscles compared to the dorsal muscle. Muscle residue depletion was below the maximum residue level from day 7 after IM injection at all three sites. These findings suggest that the cheek and pectoral fin sites provide advantages regarding systemic drug exposure and prolonged action compared with the dorsal site.
Funder
Ministry of Oceans and Fisheries
Reference24 articles.
1. Lee, J.H., Seo, J.S., Kim, G.W., Kwon, M.G., Kim, D.H., Park, C.I., Kim, K.T., and Park, J. (2022). Effect of lincomycin, an injectable lincosamide antibiotic, against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetic-pharmacodynamic profile. Aquaculture, 548.
2. Lee, J.H., Kim, G.W., Kwon, M.G., and Seo, J.S. (2021). Pharmacokinetic-Pharmacodynamic Profile, Bioavailability, and Withdrawal Time of Tylosin Tartrate Following a Single Intramuscular Administration in Olive Flounder (Paralichthys olivaceus). Animals, 11.
3. (2023, February 25). NFQS. Available online: https://www.nfqs.go.kr/apms/ebook/mice_ebook/index.html#page=1.
4. The efficacy of amoxicillin sodium against streptococcosis in cultured olive flounder Paralichthys olivaceus and its pharmacokinetics;Lim;J. Vet. Pharmacol. Ther.,2017
5. Pharmacokinetics, pharmacokinetic–pharmacodynamic relationship, and withdrawal period of amoxicillin sodium in olive flounder (Paralichthys olivaceus);Park;Xenobiotica,2016