Natural Allelic Variations of Bch10G006400 Controlling Seed Size in Chieh-qua (Benincasa hispida Cogn. var. Chieh-qua How)

Author:

Gao Yin123,Peng Jiazhu2,Qiao Yanchun12,Wang Guoping3

Affiliation:

1. Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou 510335, China

2. Guangzhou Academy of Agricultural Sciences, Guangzhou 510308, China

3. College of Horticulture, South China Agricultural University, Guangzhou 510642, China

Abstract

Seeds are the most important reproductive organs of higher plants, the beginning and end of a plant’s lifecycle. They are very important to plant growth and development, and also an important factor affecting yield. In this study, genetic analysis and BSA-seq of the F2 population crossed with the large-seeded material ‘J16’ and small-seeded material ‘FJ5’ were carried out, and the seed size locus was initially located within the 1.31 Mb region on chr10. In addition, 2281 F2 plants were used to further reduce the candidate interval to 48.8 Kb. This region contains only one gene encoding the N-acetyltransferase (NAT) protein (Bch10G006400). Transcriptome and expression analysis revealed that the gene was significantly more highly expressed in ‘J16’ than in ‘FJ5’. Variation analysis of Bch10G006400 among parents and 50 chieh-qua germplasms revealed that as well as a nonsynonymous mutation (SNP_314) between parents, two mutations (SNP_400 and InDel_551) were detected in other materials. Combining these three mutations completely distinguished the seed size of the chieh-qua. GO and KEGG enrichment analyses revealed that DGEs played the most important roles in carbohydrate metabolism and plant hormone signal transduction, respectively. The results of this study provide important information for molecular marker-assisted breeding and help to reveal the molecular mechanism of seed size.

Funder

the Special Seed Industry Revitalization Project of Rural Revitalization Strategy

the Science and Technology Program of Guangzhou

the Guangzhou Agriculture and Rural Finance Fund Project

the Guangzhou Science and Technology Planning Projects

the Agricultural Support Fund Project of Guangzhou

the Guangzhou Haizhu District Science and Technology Plan Project

Publisher

MDPI AG

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