Risks of Antibiotic Resistance Dissemination by Leachates from Municipal Landfills of Different Ages
Author:
Blumfelde Māra1, Gudrā Dita2ORCID, Začs Dzintars3, Vonda Kārlis14, Žorža Laura1, Selga Tūrs1, Grīnbergs Andrejs1, Dēliņa Aija1ORCID, Bartkevičs Vadims3ORCID, Fridmanis Dāvids2ORCID, Muter Olga1ORCID
Affiliation:
1. Faculty of Biology, University of Latvia, 1 Jelgavas Str., LV-1004 Riga, Latvia 2. Latvian Biomedical Research and Study Centre, 1 Ratsupites Str., LV-1067 Riga, Latvia 3. Institute of Food Safety, Animal Health and Environment, BIOR, 3 Lejupes Str., LV-1076 Riga, Latvia 4. Getliņi EKO Ltd., 57 Kaudzisu Str., Rumbula, LV-2121 Ropažu, Latvia
Abstract
Landfill leachate is regarded as a significant point source of pollutants that may pose a hazard to the environment, particularly to surface and ground waters. Leachates are highly variable and heterogeneous. Our study was focused on the characterization of landfill leachates derived from young (YDS) and matured (MDS) dumpsites in terms of the abundance of micropollutants, antimicrobial resistance genes (ARG), and microbial community structure. The concentrations of poly- and perfluoroalkyl substances (PFASs) were found to be higher in MDS compared to YDS, i.e., 13.19 and 7.16 μg/L, respectively. Among pharmaceutical compounds, ibuprofen was detected at the highest concentrations, i.e., 12.54, 12.81, and 13.13 μg/L, in the leachates derived from MDS, YDS, and ponds. The distribution of bacteria, archaea, eukaryotes, and viruses in the three leachate samples was as follows: 85.15 ÷ 88.04%, 9.13 ÷ 12.29%, 2.20 ÷ 2.68%, and 0.12 ÷ 0.15%, respectively. In total, 31 distinct families of ARGs were identified, comprising a total of 80 ARGs. Incubation of P. putida MSCL650 in sterile leachate from the pond resulted in decreasing the minimum inhibitory concentrations for six antibiotics as compared to cells incubated in nutrient broth. Hydrological processes, i.e., runoff and infiltration, can increase the dissemination of ARGs.
Funder
State research project in the field of biomedicine, medical technologies, and pharmacy Optimization of biotechnological processes for effective utilization of renewable resources Strengthening the capacity of the doctoral program of the University of Latvia in the framework of the new doctoral program model
Subject
Water Science and Technology,Aquatic Science,Geography, Planning and Development,Biochemistry
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