From Wisent to the Lab and Back Again—A Complex SNP Set for Population Management as an Effective Tool in European Bison Conservation

Abstract

Proper management and genetic monitoring of the modern European bison (Bison bonasus) population is one of the most important responsibilities for this species’ conservation. Up-to-date, complex genetic analysis performed using a consistent molecular method is needed for population management as a tool to further validate and maintain the genetic diversity of the species. The identification of the genetic line when pedigree data are missing, as well as the identification of parentage and individuals, are crucial for this purpose. The aim of our research was to create a small but informative panel of SNP (single-nucleotide polymorphism) markers that can be used for routine genotyping of the European bison at low cost. In our study, we used a custom-designed microarray to genotype a large number of European bison, totaling 455 samples from two genetic lines. The results of this analysis allowed us to select highly informative markers. In this paper, we present an effective single nucleotide polymorphism set, divided into separate panels to perform genetic analyses of European bison, which is needed for population monitoring and management. We proposed a total of 20 SNPs to detect hybridization with Bos taurus and Bison bison, a panel of 50 SNPs for individuals and parentage identification, as well as a panel of 30 SNPs for assessing membership of the genetic line. These panels can be used together or independently depending on the research goal and can be applied using various methods.