Author:
Galili Shmuel,Hershenhorn Joseph,Edelman Marvin,Sobolev Vladimir,Smirnov Evgeny,Amir-Segev Orit,Bellalou Aharon,Dor Evgenia
Abstract
Chickpea (Cicer arietinum L.) is an important crop in crop-rotation management in Israel. Imidazolinone herbicides have a wide spectrum of weed control, but chickpea plants are sensitive to acetohydroxyacid synthase (AHAS; also known as acetolactate synthase [ALS]) inhibitors. Using the chemical mutagen ethyl methanesulfonate (EMS), we developed a chickpea line (M2033) that is resistant to imidazolinone herbicides. A point mutation was detected in one of the two genes encoding the AHAS catalytic subunit of M2033. The transition of threonine to isoleucine at position 192 (203 according to Arabidopsis) conferred resistance of M2033 to imidazolinones, but not to other groups of AHAS inhibitors. The role of this substitution in the resistance of line M2033 was proven by genetic transformation of tobacco plants. This resistance showed a single-gene semidominant inheritance pattern. Conclusion: A novel mutation, T192I (T203I according to Arabidopsis), providing resistance to IMI herbicides but not to other groups of AHAS inhibitors, is described in the AHAS1 protein of EMS-mutagenized chickpea line M2033.
Funder
Chief Scientist of the Ministry of Agriculture.
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference55 articles.
1. Tolerance of Chickpeas to Postemergence Broadleaf Herbicides
2. Aclonifen: The identikit of a widely used herbicide;Kilinç;Afr. J. Agric. Res.,2011
3. Response of kabuli chickpea (Cicer arietinum L.) to sowing rate in Mediterranean-type environments of south-western Australia
4. Development of herbicide crops through induced mutations;Rizwan;Adv. Life Sci.,2015
5. Acetohydroxyacid synthase;Duggleby;J. Biochem. Mol. Biol.,2000
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