A New Approach to the Quantification of Fibroblast Growth Factor 23—An Array Surface Plasmon Resonance Imaging Biosensor

Author:

Tokarzewicz Anna1ORCID,Ołdak Łukasz2ORCID,Młynarczyk Grzegorz3ORCID,Klekotka Urszula4ORCID,Gorodkiewicz Ewa2

Affiliation:

1. Department of Medical Biochemistry, Medical University of Bialystok, A. Mickiewicza 2C St., 15-089 Bialystok, Poland

2. Bioanalysis Laboratory, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K St., 15-245 Bialystok, Poland

3. Department of Urology, Medical University of Bialystok, M. Sklodowskiej-Curie 24A St., 15-276 Bialystok, Poland

4. Department of Physical Chemistry, Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K St., 15-245 Bialystok, Poland

Abstract

A new biosensor based on the “surface plasmon resonance imaging (SPRi)” detection technique for the quantification of “fibroblast growth factor 23 (FGF23)” has been developed. FGF23 is mainly produced in bone tissues as a phosphaturic hormone that forms a trimeric complex with “fibroblast growth factor receptor 1 (FGFR1)” and αKlotho upon secretion. FGF23 stimulates phosphate excretion and inhibits the formation of active vitamin D in the kidneys. FGF23 has been shown to play a role in bone carcinogenesis and metastasis. The newly developed method, based on the array SPRi biosensor, was validated—the precision, accuracy, and selectivity were acceptable, and yielded less than ±10% recovery. The rectilinear response of the biosensor ranges from 1 to 75 pg/mL. The limit of detection was 0.033 pg/mL, and the limit of quantification was 0.107 pg/mL. The biosensor was used to determine FGF23 concentrations in the blood plasma of healthy subjects and patients with “clear cell” renal cell carcinoma (ccRCC). The obtained results were compared with those measured through an “enzyme-linked immunosorbent assay (ELISA)”. The determined Pearson correlation coefficients were 0.994 and 0.989, demonstrating that the newly developed biosensor can be used as a competitive method for the ELISA.

Funder

National Science Centre

EU

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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