Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri
-
Published:2023-10-23
Issue:20
Volume:24
Page:15477
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Yuan Hongbo12, Shi Bingke1, Wang Zhuoni1, Qin Genhong1, Hou Hui1, Tu Hongtao13, Wang Li1ORCID
Affiliation:
1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China 2. Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China 3. Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang 453004, China
Abstract
Valsa pyri-induced pear Valsa canker is among the most prevalent diseases to impact pear quality and yields. Biocontrol strategies to control plant disease represent an attractive alternative to the application of fungicides. In this study, the potential utility of Bacillus atrophaeus strain HF1 was assessed as a biocontrol agent against pear Valsa canker. Strain HF1 suppressed V. pyri mycelium growth by 61.20% and induced the development of malformed hyphae. Both culture filtrate and volatile organic compounds (VOCs) derived from strain HF1 were able to antagonize V. pyri growth. Treatment with strain HF1-derived culture filtrate or VOCs also induced the destruction of hyphal cell membranes. Headspace mixtures prepared from strain HF1 were analyzed, leading to the identification of 27 potential VOCs. Of the thirteen pure chemicals tested, iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibited the strongest antifungal effects on V. pyri, with respective EC50 values of 0.30, 6.65, and 74.07 μL L−1. Fumigation treatment of pear twigs with each of these three compounds was also sufficient to prevent the development of pear Valsa canker. As such, these results demonstrate that B. atrophaeus strain HF1 and the volatile compounds iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibit promise as novel candidate biocontrol agents against pear Valsa canker.
Funder
Natural Science Foundation of Xinjiang Uygur Autonomous Region Agricultural Science and Technology Innovation Program Central Public-Interest Scientific Institution Basal Research Fund Key Research and Development Program and Promotion Special Project of Henan Province
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference38 articles.
1. Selection of field control agents and comprehensive control effect of pear Valsa canker;Cao;J. Fruit,2018 2. Kange, A.M., Xia, A., Si, J., Li, B., Zhang, X., Ai, G., He, F., and Dou, D. (2020). The fungal-specific transcription factor VpFSTF1 is required for virulence in Valsa pyri. Front. Microbiol., 10. 3. Delimiting cryptic pathogen species causing apple Valsa canker with multilocus data;Wang;Ecol. Evol.,2014 4. Genome sequence of Valsa canker pathogens uncovers a potential adaptation of colonization of woody bark;Yin;New Phytol.,2015 5. Use of endophytes as biocontrol agents;Brooks;Fungal Biol. Rev.,2019
|
|