Physiological Analysis and Genetic Mapping of Short Hypocotyl Trait in Brassica napus L.

Author:

Liu Miaomiao1,Hu Fangdi1,Liu Lijun1,Lu Xiaoming1,Li Rong1,Wang Jingyu1,Wu Junyan1,Ma Li1ORCID,Pu Yuanyuan1,Fang Yan1,Yang Gang1,Wang Wangtian1,Sun Wancang1

Affiliation:

1. State Key Laboratory of Aridland Crop Science, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China

Abstract

Hypocotyl length is a botanical trait that affects the cold tolerance of Brassica napus L. (B. napus). In this study, we constructed an F2 segregating population using the cold-resistant short hypocotyl variety ‘16VHNTS158’ and the cold-sensitive long hypocotyl variety ‘Tianyou 2288’ as the parents, and BSA-seq was employed to identify candidate genes for hypocotyl length in B. napus. The results of parental differences showed that the average hypocotyl lengths of ‘16VHNTS158’ and ‘Tianyou 2288’ were 0.41 cm and 0.77 cm at the 5~6 leaf stage, respectively, after different low-temperature treatments, and ‘16VHNTS158’ exhibited lower relative ion leakage rates compared to ‘Tianyou 2288’. The contents of indole acetic acid (IAA), gibberellin (GA), and brassinosteroid (BR) in hypocotyls of ‘16VHNTS158’ and ‘Tianyou 2288’ increased with decreasing temperatures, but the IAA and GA contents were significantly higher than those of ‘Tianyou 2288’, and the BR content was lower than that of ‘Tianyou 2288’. The genetic analysis results indicate that the genetic model for hypocotyl length follows the 2MG-A model. By using SSR molecular markers, a QTL locus associated with hypocotyl length was identified on chromosome C04. The additive effect value of this locus was 0.025, and it accounted for 2.5% of the phenotypic variation. BSA-Seq further localized the major effect QTL locus on chromosome C04, associating it with 41 genomic regions. The total length of this region was 1.06 Mb. Within this region, a total of 20 non-synonymous mutation genes were identified between the parents, and 26 non-synonymous mutation genes were found within the pooled samples. In the reference genome of B. napus, this region was annotated with 24 candidate genes. These annotated genes are predominantly enriched in four pathways: DNA replication, nucleotide excision repair, plant hormone signal transduction, and mismatch repair. The findings of this study provide a theoretical basis for cloning genes related to hypocotyl length in winter rapeseed and their utilization in breeding.

Funder

Scientific Research Start-up Funds for Openly Recruited Doctors

Science and Technology Innovation Funds of Gansu Agricultural University, China

China Agriculture Research System of MOF and MARA

National Natural Science Foundation of China

Science and Technology Program of Gansu Province

Gansu Province Modern Cold and Arid Agriculture Science and Technology Support

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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