Effect of Calcium Silicate-Based Intracanal Medicament and Calcium Hydroxide on Growth Factor TGF-β1 Release from Root Canal Dentine

Author:

Bilvinaite Goda12ORCID,Drukteinis Saulius2ORCID,Simoliunas Egidijus3ORCID,Widbiller Matthias4ORCID,Sakirzanovas Simas1ORCID

Affiliation:

1. Department of Applied Chemistry, Institute of Chemistry, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania

2. Institute of Dentistry, Faculty of Medicine, Vilnius University, Zalgirio 115, LT-08217 Vilnius, Lithuania

3. Department of Biological Models, Life Science Center, Institute of Biochemistry, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania

4. Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, D-93093 Regensburg, Germany

Abstract

Effective root canal disinfection and the subsequent release of natural growth factors from dentin are crucial to the success of regenerative endodontic procedures. This study evaluated the effect of newly introduced calcium silicate-based temporary intracanal medicament Bio-C Temp and calcium hydroxide-based material UltraCal XS on the release of transforming growth factor β1 (TGF-β1) from root canal dentin. Twenty-two intact and fully developed human premolars from patients aged 15–18 were shaped and irrigated according to the current clinical recommendations. The teeth were then gently split in half, and the root canal dentin of paired samples was covered with Bio-C Temp or UltraCal XS. After 3 weeks of incubation, the specimens were conditioned with 17% EDTA and the collected solution was subjected to the quantification of the released TGF-β1 by performing an ELISA. One-way analysis of variance (ANOVA), followed by Tukey’s test, was selected to determine the statistically significant differences between the groups at the 0.95 confidence level. The highest mean value of released TGF-β1 (1993.1 pg/mL) was detected in the control group, where the root canal dentin was conditioned with 17% EDTA alone. Regarding the experimental groups, Bio-C Temp released a statistically significantly higher amount of TGF-β1 (282.14 pg/mL) compared to UltraCal XS (114.28 pg/mL; p = 0.0158). Bio-C Temp affected the release of growth factors from root canal dentin less than UltraCal XS and may therefore serve as an intracanal medicament for regenerative endodontic procedures.

Funder

Not-for-profit Kristupas Gutauskas Foundation

Publisher

MDPI AG

Reference28 articles.

1. Biomaterial scaffolds for clinical procedures in endodontic regeneration: Biomaterial scaffolds in endodontic regeneration;Liu;Bioact. Mater.,2022

2. Release of TGF- 1 into root canals with various final irrigants in regenerative endodontics;Chae;Int. Endod. J.,2018

3. In the dental implant era, why do we still bother saving teeth?;Clark;Dent. Traumatol.,2019

4. Influence of root canal disinfectants on growth factor release from dentin;Galler;J. Endod.,2015

5. Attachment and proliferation of dental pulp stem cells on dentine treated with different regenerative endodontic protocols;Alghilan;Int. Endod. J.,2017

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