Effects of Dietary Sodium Propionate on Growth, Digestive Enzyme Activity, and Expression of Immune System Genes in Juveniles of Tropical Gar (Atractosteus tropicus)

Author:

Arellano-Carrasco Jesús G.1,Martínez-García Rafael2ORCID,Asiain-Hoyos Alberto1ORCID,Reta-Mendiola Juan L.1,Díaz-Rivera Pablo1ORCID,Frías-Gómez Susana A.3,Martínez-Burguete Talhia2ORCID,Asencio-Alcudia Gloria Gertrudys24ORCID,Jiménez-Martínez Luis Daniel5,Guerrero-Zarate Rocio2,Sepúlveda-Quiroz Cesar A.2ORCID,Álvarez-González Carlos A.2ORCID

Affiliation:

1. Colegio de Postgraduados—Campus Veracruz, Km. 88.5 Carretera Federal Xalapa-Veracruz, Tepetates, Veracruz C.P. 91690, Veracruz, Mexico

2. Laboratorio de Fisiología en Recursos Acuáticos—División Académica de Ciencias Biológicas, Universidad Juárez Autónoma de Tabasco, Carretera Villahermosa-Cárdenas Km. 0.5, Villahermosa C.P. 86039, Tabasco, Mexico

3. Posgrado en Ciencias del Mar y Limnología (Posgrado en Ciencias del Mar y Limnología), Universidad Nacional Autónoma de Mexico, Av. Universidad 3000, Ciudad Universitaria Coyoacán, Ciudad de México C.P. 04510, Mexico City, Mexico

4. Tecnológico Nacional de México Campus Villahermosa, Km. 3.5 Carretera Villahermosa–Frontera, Cd. Industrial, Villahermosa C.P. 86010, Tabasco, Mexico

5. Laboratorio de Biología Molecular, División Académica Multidisciplinaria de Jalpa de Méndez, Universidad Juárez Autónoma de Tabasco, Carretera Estatal Libre Villahermosa-Comalcalco Km. 27 S/N, Jalpa de Méndez C.P. 86205, Tabasco, Mexico

Abstract

We determined the effects of sodium propionate (SP) added to the diets of Atractosteus tropicus juveniles with respect to the growth, survival, digestive enzyme activity, and expression of genes that are associated with the immune system. Five treatments (0, 0.5, 1.0, 1.5, and 2.0%) were evaluated in triplicate on 180 fish (3.65 ± 0.12 g) distributed among 15 (70 L) tanks. The juveniles were fed five times a day with 5% feed in relation to the biomass of the organism. The treatment with 0.5% SP showed a final weight value of 25.7 ± 4.5 g, absolute weight of gain (AWG) of 21.93 ± 4.39 g, and specific growth rate (SGR) of 3.1 ± 0.26. Treatments with 1.5 and 2.0% SP showed the highest survival (91.6%). The control group (0%) showed a greater activity of lipases. There was a tendency that the highest activity of alkaline proteases and chymotrypsin occurred in the 0 and 0.5% treatments. The maximum relative expression of the genes ocln, muc2, and nod2 occurred in the 1.5% treatment. The inclusion of SP in the diet of A. tropicus juveniles could benefit the activity of some digestive enzymes as well as the expression of genes related to the function of the intestinal barrier, therefore benefitting the survival of the organisms.

Funder

Consejo Nacional de Ciencia y Tecnología

Colegio de Postgraduados Campus Veracruz

Publisher

MDPI AG

Subject

General Medicine

Reference48 articles.

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2. Estado de arte de la biología y cultivo de pejelagarto (Atractosteus tropicus);Agroproductividad,2015

3. Evaluation of Mannan oligosaccharides (MOS) in balanced diets for tropical gar juveniles (Atractosteus tropicus);Gisbert;Hidrobiologica,2018

4. Nieves-Rodríguez, K.N., Álvarez-González, C.A., Peña-Marín, E.S., Vega-Villasante, F., Martínez-García, R., Camarillo-Coop, S., Tovar-Ramírez, D., Guzmán-Villanueva, L.T., Andree, K.B., and Gisbert, E. (2018). Effect of β-Glucans in Diets on Growth, Survival, Digestive Enzyme Activity, and Immune System and Intestinal Barrier Gene Expression for Tropical Gar (Atractosteus tropicus) Juveniles. Fishes, 3.

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