Affiliation:
1. International Center for Bamboo and Rattan, Key Laboratory of National Forestry and Grassland Administration, Beijing 100102, China
2. School of Karst Science, Guizhou Normal University, Guiyang 550001, China
Abstract
Rational application of N fertilizer is essential for maintaining the long-term productivity of Moso bamboo forests. Microbial activity is a crucial indicator of soil quality. Changes in soil nutrient resources due to N addition can lead to microbial nutrient limitations, thereby impeding the maintenance of soil quality. Currently, there is limited research on the effects of N application on microbial nutrient limitations in Moso bamboo forest soils. To examine the changes in extracellular enzyme activity and microbial nutrient limitations in Moso bamboo forest soils following N application, we conducted an N application experiment in northern Guizhou. The findings revealed that the N3 treatment (726 kg·N·hm−2·yr−1) significantly reduced β-glucosidase (BG) activity by 27.61% compared to the control group (no fertilization). The N1 (242 kg·N·hm−2·yr−1), N2 (484 kg·N·hm−2·yr−1), and N3 treatments notably increased the activities of leucine aminopeptidase (LAP) and N-acetyl-β-D-glucosidase (NAG) by 11.45% to 15.79%. Acid phosphatase (ACP) activity remained unaffected by fertilization. N application treatments significantly decreased the C:Ne and C:Pe ratios, while the N:Pe ratio was less influenced by N fertilizer application. Scatter plots and vector characteristics of enzyme activity stoichiometry suggested that microorganisms in the study area were limited by C and N, and N fertilizer application reduced the vector length and increased the vector angle, indicating that N application alleviated the C and N limitation of microorganisms in Moso bamboo forests. Redundancy Analysis (RDA) demonstrated that microbial biomass phosphorus (MBP) was the most critical factor affecting extracellular enzyme activity and stoichiometry. Furthermore, Random Forest Regression analysis identified MBP and the N:Pm ratio as the most significant factors influencing microbial C and N limitation, respectively. The study demonstrated that N application modulates the microbial nutrient acquisition strategy by altering soil nutrient resources in Moso bamboo forests. Formulating fertilizer application strategies based on microbial nutrient requirements is more beneficial for maintaining soil quality and sustainably managing Moso bamboo forests. Additionally, our study offers a theoretical reference for understanding carbon cycling in bamboo forest ecosystems in the context of substantial N inputs.
Funder
National Key R&D Program of China of the 14th Five-Year Plan
New Agricultural Science Research and Reform Practice Project
Reference71 articles.
1. Jiang, Z. (2002). Bamboo and Rattan in the World, China Forest Publishing House.
2. Nitrogen Addition Increased CO2 Uptake More than Non-CO2 Greenhouse Gases Emissions in a Moso Bamboo Forest;Song;Sci. Adv.,2020
3. China’s Bamboo Resources in 2021;Feng;World Bamboo Ratt.,2023
4. Calculation of carbon sink of bamboo forest in China and its potential prediction;Fu;China For. Econ.,2023
5. Changes of Carbon Stocks in Bamboo Stands in China during 100 Years;Chen;For. Ecol. Manag.,2009
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献