The Influence of Betulin and Its Derivatives on Selected Colorectal Cancer Cell Lines’ Viability and Their Antioxidant Systems-Reference-Cited by-同舟云学术

The Influence of Betulin and Its Derivatives on Selected Colorectal Cancer Cell Lines’ Viability and Their Antioxidant Systems

Published:2024-08-17 Issue:16 Volume:13 Page:1368
ISSN:2073-4409
Container-title:Cells
language:en
Short-container-title:Cells

Author:

Madej Marcel¹^ORCID,Kruszniewska-Rajs Celina¹^ORCID,Kimsa-Dudek Magdalena²^ORCID,Synowiec-Wojtarowicz Agnieszka²^ORCID,Chrobak Elwira³^ORCID,Bębenek Ewa³^ORCID,Boryczka Stanisław³^ORCID,Głuszek Stanisław⁴⁵^ORCID,Adamska Jolanta¹,Kubica Sebastian¹^ORCID,Matykiewicz Jarosław⁴⁵,Gola Joanna Magdalena¹^ORCID

Affiliation:

1. Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland

2. Department of Nutrigenomics and Bromatology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland

3. Department of Organic Chemistry, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland

4. Department of Surgical Medicine with the Laboratory of Medical Genetics, Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University, 25-317 Kielce, Poland

5. Department of Clinic Oncological Surgery Holycross Center, 25-317 Kielce, Poland

Abstract

Oxidative stress is considered one of the main reasons for the development of colorectal cancer (CRC). Depending on the stage of the disease, variable activity of the main antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), is observed. Due to limited treatment methods for CRC, new substances with potential antitumor activity targeting pathways related to oxidative stress are currently being sought, with substances of natural origin, including betulin, leading the way. The betulin molecule is chemically modified to obtain new derivatives with improved pharmacokinetic properties and higher biological activity. The aim of this study was to evaluate the effects of betulin and its new derivatives on viability and major antioxidant systems in colorectal cancer cell lines. The study showed that betulin and its derivative EB5 affect the antioxidant enzyme activity to varying degrees at both the protein and mRNA levels. The SW1116 cell line is more resistant to the tested compounds than RKO, which may be due to differences in the genetic and epigenetic profiles of these lines.

Funder

Medical University of Silesia

program of the Minister of Education and Science called “Regional Initiative of Excellence”

Publisher

MDPI AG

Link

https://www.mdpi.com/2073-4409/13/16/1368/pdf

Reference45 articles.

1. Early-onset colorectal cancer: A review of current knowledge;Saraiva;World J. Gastroenterol.,2023

2. Current and emerging therapeutic approaches for colorectal cancer: A comprehensive review;Kumar;World J. Gastrointest. Surg.,2023

3. Underwood, P.W., Ruff, S.M., and Pawlik, T.M. (2024). Update on targeted therapy and immunotherapy for metastatic colorectal cancer. Cells, 13.

4. Amiri, S., Dastghaib, S., Ahmadi, M., Mehrbod, P., Khadem, F., Behrouj, H., Aghanoori, M.R., Machaj, F., Ghamsari, M., and Rosik, J. (2020). Betulin and its derivatives as novel compounds with different pharmacological effects. Biotechnol. Adv., 38.

5. Madej, M., Gola, J., and Chrobak, E. (2023). Synthesis, pharmacological properties, and potential molecular mechanisms of antitumor activity of betulin and its derivatives in gastrointestinal cancers. Pharmaceutics, 15.