Abstract
Arboviruses have been reported over the years as constant threats to blood transfusion recipients, given the high occurrence of asymptomatic cases and the fact that the presence of viremia precedes the onset of symptoms, making it possible that infected blood from donors act as a source of dissemination. This work aims to identify the prevalence of dengue virus (DENV), Zika virus (ZIKV) and Chikungunya virus (CHIKV) infection in blood donors during epidemic and non-epidemic periods; classify the donor as symptomatic or asymptomatic; and verify the need to include DENV, CHIKV and ZIKV in the nucleic acid test (NAT) platform in northern Brazil. We investigated 36,133 thousand donations in two years of collection in Northern Brazil. One donor was positive for DENV and one for CHIKV (0.002% prevalence). As the prevalence for arboviruses was low in this study, it would not justify the individual screening of samples from donors in a blood bank. Thus, DENV- and CHIKV-positive samples were simulated in different amounts of sample pools, and both were safely detected by molecular biology even in a pool of 14 samples, which would meet the need to include these three viruses in the routine of blood centers in endemic countries such as Brazil.
Funder
Brazilian funding agencies National Counsel of Technological and Scientific Development
Fundação Amazônia Paraense de Amparo à Pesquisa
Pró-Reitoria de Pesquisa e Pós-Graduação (PROPESP) of Universidade Federal do Pará
Reference35 articles.
1. Detection of dengue, chikungunya, and Zika RNA in blood donors from Southeast Asia;Stanley;Transfusion,2021
2. Detection of dengue virus in sera of Brazilian blood donors;Dias;Transfusion,2012
3. Frequency of dengue virus infection in blood donors in Sistan and Baluchest an province in Iran;Aghaie;Transfus. Apher. Sci.,2014
4. Dengue virus life cycle: Viral and host factors modulating infectivity;Wilschut;Cell Mol. Life Sci.,2010
5. Grard, G., Caron, M., Mombo, I.M., Nkoghe, D., Mboui Ondo, S., Jiolle, D., Fontenille, D., Paupy, C., and Leroy, E.M. (2014). Zika virus in Gabon (Central Africa)—2007: A new threat from Aedes albopictus?. PLoS Negl. Trop. Dis., 8.